Objective: To observe the expression and role of long non-coding RNA(LncRNA) potassium voltage-gated channel subfamily q member 1 overlapping transcription 1(KCNQ1OT1) in coronary atherosclerotic heart disease(CHD), and to explore whether it can become a potential biomarker for predicting acute myocardial infarction(AMI). Methods: A total of 240 patients were selected and divided into AMI group, unstable angina pectoris(UAP) group, stable angina pectoris(SAP) group and non-coronary heart disease control group, with 60 cases in each group. Venous blood was collected from the AMI group 6 hours after symptom onset, and blood samples were collected from the other three groups in the early morning of the next day after admission. The supernatant of the blood samples was extracted after centrifugation and frozen for RNA extraction. The basic clinical data, myocardial injury markers, lipid levels and other test results of the selected patients were recorded, and their history of hypertension and diabetes was noted. The expression levels of LncRNA in the peripheral blood of the patients were detected by real-time fluorescence quantitative PCR(RT-qPCR), and the ROC curve was drawn and the area under the curve was calculated to determine the diagnostic value of LncRNA. The correlation between LncRNA and some myocardial injury markers, N-terminal pro-B-type natriuretic peptide(NT-proBNP), and left ventricular ejection fraction(LVEF) was analyzed. Results: (1) The expression of LncRNA KCNQ1OT1 in the serum of AMI patients was significantly higher than that in the control group(P＜0.05).(2) The expression of LncRNA KCNQ1OT1 in the serum of AMI patients was positively correlated with the expression of cTnI, CK-MB and NT-proBNP, and negatively correlated with the expression of LVEF%.(3) The predictive ability of LncRNA KCNQ1OT1: AUC was 0.793(95% CI: 0.727 - 0.859), with a sensitivity of 60.2%, a specificity of 90.2%, and a cutoff value of 1.635. Conclusion: The highly expressed LncRNA KCNQ1OT1 in serum can serve as a biological marker for the diagnosis and prognosis assessment of AMI.
CHEN Xiaolin
,
LI Meng
,
HOU Feng
. The expression and role of LncRNA KCNQ1OT1 in coronary atherosclerotic heart disease[J]. Journal of Baotou Medical College, 2025
, 41(10)
: 52
-58
.
DOI: 10.16833/j.cnki.jbmc.2025.10.010
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