Objective: To study the effects of different concentrations of D-ribose on the proliferative viability, intracellular ATP level and mitochondrial respiratory chain-related gene expression of K562 cells. Methods: K562 cells were incubated with different concentrations of D-ribose and D-glucose in basal medium or PBS for 6, 24 or 48 hours, and the proliferation viability, intracellular ATP level and the expression of genes related to mitochondrial respiratory chain (MTND6, MTATP8) were detected by using CCK-8, ATP detection kit and real-time fluorescence quantitative PCR (RT-qPCR), respectively. Results: Unlike the proliferative effect induced by D-glucose, low concentrations of D-ribose (10-25 mmol/L) had no significant effect on cell proliferation within 24 hours, but significantly inhibited cell proliferation after 48 hours. The results of ATP detection showed that the ATP level was generally increased in the basal medium for 24 h, which was different from that in the glucose group, and only the ATP level was increased in the 50 mmol/L D-ribose group; after 48 h, although the ATP in the control group was almost depleted, the ATP in the 10 mmol/L and 20 mmol/L ribose groups was significantly higher than that in the control group. Under the condition of sugar-free (PBS), unlike D-glucose, which significantly increased ATP levels, D-ribose could not effectively maintain ATP levels. RT-QPCR showed that D-ribose significantly inhibited the expression of mitochondrial respiratory chain gene MTATP8 (P＜0.01), but had no effect on the expression of MTND6. Conclusion: High concentration of D-ribose can inhibit the proliferation of K562 cells and the expression of respiratory chain genes, and has little contribution to the promotion of ATP production, but may reduce the utilization of ATP. This study lays an experimental foundation for further understanding the function of D-ribose in maintaining metabolic homeostasis.
WANG Zeying
,
YANG Zhongbin
,
LI Houyu
,
HE Qi
,
JIN Jing
,
LIU Zhi
,
LI Xiaojing
,
SU Yan
. Effects of D-ribose on proliferation and energy metabolism of K562 cells[J]. Journal of Baotou Medical College, 2025
, 41(9)
: 20
-24
.
DOI: 10.16833/j.cnki.jbmc.2025.09.004
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