Objective: To study the core components and mechanism of sea buckthorn flavonoids anti-atherosclerosis (anti-AS) by network pharmacology and molecular docking technology. Methods: The active components and targets of Sea buckthorn were searched by TCMSP database, AS targets were collected by Genecards and OMIM database, intersection targets were obtained by Venny 2.1, and PPI networks were mapped by Cytoscape 3.7.2. GO function analysis and KEGG pathway enrichment analysis were performed on the selected targets using Metascape platform. Finally, AutoDock and PyMOL software were used for molecular docking. Results: 11 active ingredients, 224 targets and 80 intersection targets with AS were selected. A total of 5 242 items were identified by GO functional analysis, and 234 pathways were enriched by KEGG pathway analysis, among which fluid shear stress and atherosclerosis pathway, PI3K-Akt signaling pathway and VEGF signaling pathway were key pathways. Molecular docking results showed that the main active components of sea buckthorn quercetin, isorhamnetin and stigasterol had good docking with the target IL1β, PPARG, ADRB2 and NOS3. Conclusion: Quercetin and isorhamnetin in sea buckthorn may act on IL1β, PPARG, ADRB2, NR3C1 and NOS3 targets of fluid shear stress and atherosclerosis pathway, PI3K-Akt signaling pathway and VEGF signaling pathway, thus, it can achieve anti-AS effect by anti-inflammation, regulating oxidative stress, lipid metabolism disorders, and repairing vascular damage, etc., which provides a theoretical basis for subsequent basic research.
WANG Xiaoxue
,
YU Jianbin
,
LIU Wanyu
,
WANG Yan
,
WANG Shunzhe
,
ZHAO Jing
,
TIAN Xinyi
,
XUE Yongzhi
,
ZHANG Dong
. To explore the molecular mechanism of Sea buckthorn flavonoids against atherosclerosis based on network pharmacology[J]. Journal of Baotou Medical College, 2025
, 41(8)
: 22
-27
.
DOI: 10.16833/j.cnki.jbmc.2025.08.005
[1] 国家药典委员会.中华人民共和国药典: 一部[M]. 北京: 中国医药科技出版社, 2020.
[2] 田茜, 何晨, 贺敬霞, 等. 沙棘总黄酮-聚乙烯吡咯烷酮K30固体分散体的制备, 表征及体外溶出研究[J].中国药房, 2017, 28(1): 115-118.
[3] Pop RM, Socaciu C, Pintea A, et al. UHPLC/PDA-ESI/MS analysis of the main berry and leaf flavonol glycosides from different Carpathian Hippophaë rhamnoides L. varieties[J]. Phytochem Anal, 2013, 24(5): 484-492.
[4] 张东, 邬国栋. 沙棘黄酮的化学成分及药理作用研究进展[J]. 中国药房, 2019, 30(9): 1292-1296.
[5] Nelson AJ, Bubb K, Nicholls SJ. An update on emerging drugs for the treatment of hypercholesterolemia[J]. Expert Opin Emerg Drugs, 2021, 26(4): 363-369.
[6] 吴希泽, 康健, 李越, 等. 基于“浊气归心, 淫精于脉”理论运用HPLC-Q-TOF-MS/MS和网络药理学探讨抵挡汤防治动脉粥样硬化和高脂血症的作用机制[J]. 中国中药杂志, 2023, 48(5): 1352-1369.
[7] Lara-Guzman OJ, Tabares-Guevara JH, Leon-Varela YM, et al. Proatherogenic macrophage activities are targeted by the flavonoid quercetin[J]. J Pharmacol Exp Ther, 2012, 343(2): 296-306.
[8] Luo Y, Sun G, Dong X, et al. Isorhamnetin attenuates atherosclerosis by inhibiting macrophage apoptosis via PI3K/AKT activation and HO-1 induction[J]. PLoS One, 2015, 10(3): e0120259.
[9] Feng S, Dai Z, Liu AB, et al. Intake of stigmasterol and β-sitosterol alters lipid metabolism and alleviates NAFLD in mice fed a high-fat western-style diet[J]. Biochim Biophys Acta Mol Cell Biol Lipids, 2018, 1863(10): 1274-1284.
[10] Jayaraman A, Htike TT, James R, et al. TNF-mediated neuroinflammation is linked to neuronal necroptosis in Alzheimer's disease hippocampus[J]. Acta Neuropathol Commun, 2021, 9(1): 159.
[11] Ren K, Li H, Zhou HF, et al. Mangiferin promotes macrophage cholesterol efflux and protects against atherosclerosis by augmenting the expression of ABCA1 and ABCG1[J]. Aging (Albany NY), 2019, 11(23): 10992-11009.
[12] Camps SGJA, Verhoef SPM, Bouwman FG, et al. Association of FTO and ADRB2 gene variation with energy restriction induced adaptations in resting energy expenditure and physical activity[J]. Gene X, 2019, 18(3): 100019.
[13] 李静雅. 糖皮质激素受体基因多态性与脑小血管病的相关性研究[D]. 唐山: 华北理工大学, 2019.
[14] 孙婷, 池晴佳, 王贵学. NOS3参与心血管疾病调控机制研究进展[J].生物医学工程学杂志, 2016, 33(1): 188-192.
[15] 曹珊, 张艺嘉, 白杨, 等. 基于消斑通脉方抗动脉粥样硬化作用机制的网络药理学分析和体外实验验证[J].吉林大学学报(医学版), 2024, 50(4): 925-938.
[16] Baeyens N, Bandyopadhyay C, Coon BG, et al. Endothelial fluid shear stress sensing in vascular health and disease[J]. J Clin Invest, 2016, 126(3): 821-828.
[17] Ma L, Zhang R, Li D, et al. Fluoride regulates chondrocyte proliferation and autophagy via PI3K/AKT/mTOR signaling pathway[J]. Chem Biol Interac, 2021, 349: 109659.
[18] Lu XL, Zhao CH, Yao XL, et al. Quercetin attenuates high fructose feeding-induced atherosclerosis by suppressing inflammation and apoptosis via ROS-regulated PI3K/AKT signaling pathway[J]. Biomed Pharmacother, 2017, 85: 658-671.
[19] Jia Q, Cao H, Shen D, et al. Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1[J]. Int J Mol Med, 2019, 44(3): 893-902.
[20] Luo Y, Sun G, Dong X, et al. Isorhamnetin attenuates atherosclerosis by inhibiting macrophage apoptosis via PI3K/AKT activation and HO-1 induction[J]. PLoS One, 2015, 10(3): e0120259.
[21] Liu J, Xu P, Liu D, et al. TCM regulates PI3K/Akt signal pathway to intervene atherosclerotic cardiovascular disease[J]. Evid Based Complement Alternat Med, 2021, 2021(1): 4854755.
