Objective: To investigate the value of serum and gingival crevicular fluid myeloid cell-associated protein-8/14 (MRP-8/14), Toll-like receptor 4 (TLR4), Toll-like receptor 9 (TLR9) in evaluating periodontal-orthodontic efficacy in periodontitis patients with malocclusion. Methods: A total of 72 periodontitis patients with malocclusion admitted to Yongcheng People's Hospital from January 2022 to July 2023 were selected as the observation group, and 72 healthy subjects in the same period were selected as the control group. The levels of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid were compared between the two groups before treatment. The indexes of serum and gingival crevicular fluid, inflammatory response indexes [high-sensitivity C-reactive protein (hs-CRP), soluble intercellular adhesion molecule (sICAM-1), monocyte chemoattractant protein-1 (MCP-1)] and periodontal indexes [plaque index (PLI), bleeding index (SBI), probing depth (PD)] were compared between the two groups before treatment and 3 months after treatment. The relationship between the indexes of serum and gingival crevicular fluid and the indexes of inflammatory response and periodontal indexes and the evaluation value were analyzed. Results: Before treatment, the levels of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid in the observation group were higher than those in the control group(P＜0.05). The levels of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid of patients with good efficacy and poor efficacy were significantly lower than those before treatment after 3 months of treatment (P＜0.05). The levels of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid of patients with good efficacy were significantly lower than those of patients with poor efficacy before treatment and after 3 months of treatment (P＜0.05). The levels of SBI, PLI, PD, serum hs-CRP, sICAM-1 and MCP-1 in patients with good efficacy before treatment in the observation group were lower than those in patients with poor efficacy. MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid were positively correlated with hs-CRP, PLI, sICAM-1, SBI, MCP-1 and PD (P＜0.05). The AUC of combined evaluation of serum and gingival crevicular fluid indexes before treatment and 3 months after treatment was the largest (P＜0.05). Conclusion: The high expression of MRP-8/14, TLR4 and TLR9 in serum and gingival crevicular fluid of periodontitis patients with malocclusion is related to inflammatory response, periodontal index and periodontal-orthodontic efficacy. The combined prediction of periodontal-orthodontic efficacy has a high reference value.
ZHAO Zhen
,
SHI Sumin
,
Gulibaha·Maimaitili
,
ZHANG Juanjuan
. The value of MRP-8/14, TLR4 and TLR9 in evaluating the periodontal-orthodontic effect of periodontitis patients with malocclusion[J]. Journal of Baotou Medical College, 2025
, 41(6)
: 79
-85
.
DOI: 10.16833/j.cnki.jbmc.2025.06.015
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