Objective: To observe the effect of Mongolian medicine total flavonoids of Dracocephalum moldavica L (TFDM) on the expression of microtubule-associated protein-2 (MAP-2) in the ischemic penumbra of rats after cerebral ischemia-reperfusion, and explore its role in neural plasticity. Methods: Constructing a middle cerebral artery occlusion reperfusion model using SD rats. The experiment was divided into sham surgery group (Sham), ischemia-reperfusion group (I/R), and TFDM administration group (TFDM). Samples were collected at four time points: 1, 3, 7, 14 days after surgery. The immunohistochemical staining was used to observe the expression of MAP-2 positive labeled cells and dendrites in the ischemic penumbra of the cortex, and Western blot was used to detect the expression of MAP-2 protein in the ischemic penumbra of the cortex. Results: The results of immunohistochemistry and Western Blot showed that the expression of MAP-2 in the ischemic penumbra of the TFDM group was significantly higher than that in the I/R group on the 3rd and 7th day after reperfusion (P＜0.05). Conclusion: TFDM may play a neuroprotective role by up-regulating the expression of MAP-2 protein, enhancing the regulation of neural plasticity in the ischemic penumbra, promoting dendritic growth and repairing damaged nerve structure.
ZHAI Weikai
,
CONG Wei
,
GAO Tianyi
,
CUI Chengli
,
LI Liang
. Effect of total flavonoids of Dracocephalum moldavica L. on the expression of microtubule-associated protein-2 in ischemic penumbra of rats after cerebral ischemia-reperfusion[J]. Journal of Baotou Medical College, 2025
, 41(6)
: 43
-48
.
DOI: 10.16833/j.cnki.jbmc.2025.06.009
[1] Zhao YF, Zhang XJ, Chen XY, et al. Neuronal injuries in cerebral infarction and ischemic stroke: From mechanisms to treatment (Review)[J]. Int J Mol Med, 2022,49(2):15.
[2] 杨琼英, 冯晋, 宁珑, 等. 脑缺血再灌注损伤模型大鼠脑组织缺血半暗带病理进程研究[J]. 药学研究, 2021, 40(8):491-496, 507.
[3] 姚中凯, 吴作培, 孙贵新. 微管相关蛋白2:调节神经元发育、结构稳定及突起形成和突触可塑性[J]. 中国组织工程研究, 2015, 19(37):6010-6016.
[4] 刘云, 靳敏, 王占黎. 香青兰总黄酮化学成分和药理作用研究进展[J]. 中国民族民间医药, 2019, 28(1):68-71.
[5] 孙雅煊, 刘婷, 戴雪伶, 等. 香青兰总黄酮对短暂性脑缺血诱导的炎症反应的影响[J]. 生物物理学报, 2012, 28(6):469-476.
[6] 李梁, 蔡志平, 白雪梅, 等. 香青兰总黄酮促进脑缺血再灌注大鼠室管膜下区巢蛋白表达[J]. 神经解剖学杂志, 2020, 36(6):633-640.
[7] 陈丽, 曹星月, 王占黎, 等. 内蒙古香青兰总黄酮提取工艺优化及其抗氧化活性成分分析[J]. 中国现代应用药学, 2023, 40(9):1187-1193.
[8] Wu P, Yan XS, Zhang Y, et al. The protective mechanism underlying total flavones of Dracocephalum (TFD) effects on rat cerebral ischemia reperfusion injury[J]. J Toxicol Environ Health A, 2018,81(23):1199-1206.
[9] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989,20(1):84-91.
[10] 曹香玲, 吴新贵, 张俊川, 等. 电针治疗对MCAO大鼠运动功能及梗死对侧大脑皮层GAP-43、MAP-2表达的影响[J]. 华中科技大学学报(医学版), 2018, 47(3):274-279.
[11] 张勇, 付雪琴, 邹旭欢, 等. 急性脑缺血/再灌注损伤后神经元细胞骨架蛋白的动态变化[J]. 中国药理学通报, 2024, 40(2):263-272.
[12] Mages B, Fuhs T, Aleithe S, et al. The cytoskeletal elements MAP2 and NF-L show substantial alterations in different stroke models while elevated serum levels highlight especially MAP2 as a sensitive biomarker in stroke patients[J]. Mol Neurobiol, 2021,58(8):4051-4069.
[13] Li Y, Jiang N, Powers C, et al. Neuronal damage and plasticity identified by microtubule-associated protein 2, growth-associated protein 43, and cyclin D1 immunoreactivity after focal cerebral ischemia in rats[J]. Stroke, 1998,29(9):1972-1980;discussion 1980-1981.
[14] Zhang YQ, Li H, Huang M, et al. Neuroprotective effects of Gualou Guizhi decoction in vivo and in vitro[J]. J Ethnopharmacol, 2014,158 Pt A:76-84.
[15] Wang FJ, Li RY, Tu PF, et al. Total glycosides of Cistanche deserticola promote neurological function recovery by inducing neurovascular regeneration via nrf-2/keap-1 pathway in MCAO/R rats[J]. Front Pharmacol, 2020,11:236.
[16] 龙建飞, 张秋霞, 张建, 等. 黄连解毒汤有效部位对脑缺血大鼠神经元MAP-2、PGP9.5表达及星形胶质细胞活化的影响[J]. 中医药信息, 2014, 31(5): 49-53.
[17] 袁娅金, 张桂仙, 冉利, 等. 脑卒中后神经可塑性相关信号通路的研究进展[J]. 中华老年心脑血管病杂志, 2020, 22(1):106-108.
