Objective: To explore the effect of glycopyrronium bromide on lung ischemia-reperfusion injury (LIRI) in rats, and to analyze the mechanism of HMGB1/TLR4/NF-κB signaling pathway in this process, so as to provide theoretical basis for clinical application. Methods: Thirty-two 6-week-old male SD rats were randomly divided into 4 different groups, with 8 rats in each group. Four groups were: sham operation group (Sham), ischemia reperfusion injury model group (I/R), low dose group (L) and high dose group (H). The pathological changes of lung tissue were observed and evaluated by HE staining. The ratio of wet weight to dry weight (W/D) was measured to determine the water content of lung tissue. The levels of indicators related to oxidative stress in lung tissue were detected. At the same time, the expression of HMGB1, TLR4 and p-NF-κB protein in lung tissue was analyzed by Western blotting, and the expression levels of HMGB1, TLR4 and NF-κB mRNA were detected by RT-qPCR. Results: Pathological findings showed that the lung tissue structure of the Sham group was clear and no damage was observed; while in the I/R group, pulmonary capillary congestion, interstitial edema, alveolar wall thickening, severe lung tissue damage, and a large number of inflammatory cell infiltration were observed. The above conditions in group L and group H were less than those in the I/R group, and H group was lighter. Compared with Sham group, the W/D ratio and the expression levels of HMGB1, TLR4 and NF-κB mRNA in the I/R group and L group were increased. However, compared with the I/R group, the performance of the above indicators in the L group and H group decreased, and the data in the H group were lower, the differences were statistically significant (P＜0.05). Further analysis showed that compared with Sham group, the concentration of malondialdehyde (MDA), the expression of HMGB1, TLR4 and p-NF-κB protein in the I/R group, L group and H group increased, while the activity of superoxide dismutase (SOD) decreased. Compared with the I/R group, the expression of the above indicators in the L group and H group decreased, while the SOD activity increased, and the performance of the H group was more obvious, the differences were statistically significant (P＜0.05). Conclusion: Glycopyrronium bromide can reduce the inflammatory response and oxidative stress during LIRI by inhibiting the activation of HMGB1/TLR4/NF-κB signaling pathway, thereby improving LIRI in rats.
KANG Jian
,
GAO Manhai
,
CAO Lirong
,
CHEN Zhiqiang
,
WU Juan
. Glycopyrronium Bromide improves lung ischemia-reperfusion injury by regulating HMGB1/TLR4/NF-κB signaling pathway[J]. Journal of Baotou Medical College, 2025
, 41(6)
: 37
-42
.
DOI: 10.16833/j.cnki.jbmc.2025.06.008
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