Objective: To investigate the changes of tachykinin 1 (Tac1) neurons in central amygdala (CeA) of acute pain model mice. Methods: The experimental animals were divided into saline group, formalin group and capsaicin (Cap) group by random number table method. Male C57BL/6 mice were used to prepare acute pain model. 20 μL Formalin and Cap were injected into the left hind paw of mice, and the control group was injected with the same volume of normal saline. The positive expression of c-FOS in CeA of mice was observed 2 h after successful modeling. The activation of CeATac1 neurons in two acute pain models was observed by immunofluorescence histochemistry. The images were collected and the neurons were counted and analyzed. Results: The number of c-FOS positive neurons activated in CeA in formalin group and Cap group was higher than that in control group (P＜0.000 1), and the number of c-FOS positive neurons in formalin group was higher than that in Cap group (P＜0.01). The proportion of c-FOS/tdTomato double-labeled neurons in CeATac1 neurons in Formalin group and Cap group was (3.50±0.71)% and (1.10±0.32)%, respectively, and that in Formalin group was higher than that in Cap group (P＜0.000 1). Conclusion: The CeATac1 neurons can be activated under both acute pain stimuli, the CeATac1 neurons are involved in the conduction of acute pain, and c-FOS expression in CeATac1 neurons is more obvious under Formalin stimulation.
ZHANG Yingning
,
KOU Zhenzhen
,
CAI Zhiping
. Changes of Tac1 neurons in the central amygdaloid nucleus of mice in acute pain model[J]. Journal of Baotou Medical College, 2025
, 41(5)
: 53
-57
.
DOI: 10.16833/j.cnki.jbmc.2025.05.010
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