Objective: To investigate the molecular mechanism by which exosomal secretion of miR-133a-3p regulates TGFBR1 and promotes the progression of prostate cancer. Methods: Exosomes were extracted from prostate cancer cells, and the expression of exosom-associated markers such as leukocyte differentiation antigen 81 (CD81) and leukocyte differentiation antigen 63 (CD63) was detected by Western blot assay (WB). The expression of miR-133a-3p was detected by miRNA matrix sequencing. Cell cloning experiment, cell proliferation 8 (CCK-8) experiment, EdU cell proliferation experiment and cell migration experiment were used to detect the effects of co-cultured exosomes+miR-133a-3p on the proliferation and metastasis of prostate cancer. Dual luciferase reporter gene assay was used to detect the binding of miR-133a-3p to TGFBR1. Real-time fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) and WB were used to detect the effect of regulation of miR-133a-3p on transforming growth factor β receptor 1 (TGFBR1) . Results: Compared with NC group, the expressions of CD81 and CD63 in co-cultured exosomes were increased (P＜0.05). Cell cloning experiment, CCK-8 experiment, EDU experiment, scratch experiment, and cell migration experiment showed that compared with NC group, the cell proliferation and metastasis ability of Mir-133a-3P group (transfected with miR-133a mimic) decreased (P＜0.05). Dual luciferase reporter gene experiment showed that miR-133a-3p binding TGFBR1 sequence and overexpression of miR-133a-3p inhibited the expression of TGFBR1 (P＜0.05). WB results showed that compared with NC group, the expressions of WNT, GSK3β, C-MYC and β-catenin in si-TGFBR1 group were decreased, while the expressions of WNT, GSK3β, C-MYC and β-catenin in oe-TGFBR1 group were increased (P＜0.05). However, the expressions of WNT, GSK3β, C-MYC and β-catenin in oe-TGFBR1+miR-133a-3p group were not different from those in NC group. Conclusion: Prostate cancer cell-derived exosome miR-133a-3p targets TGFBR1 to promote the malignant phenotype of prostate cancer cells.
WU Hongliang
,
WANG Sheng
,
CHEN Zhijun
,
YANG Shuai
,
SUN Wenyan
,
GUAN Han
. Prostate cancer cell derived exosome miR-133a-3p promotes the malignant phenotype of protate cancer cells by targeting TGFBR1[J]. Journal of Baotou Medical College, 2025
, 41(5)
: 42
-47
.
DOI: 10.16833/j.cnki.jbmc.2025.05.008
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