Objective: To investigate the effects of human umbilical cord mesenchymal stem cells on renal tissue injury and expression of uromodulin in rats with diabetic nephropathy. Methods: The diabetic nephropathy model was established and divided into normal control group (NC group), diabetic kidney disease (DKD) group (DKD group), and human umbilical cord mesenchymal stem cells (HU-MSCs) treatment of diabetic nephropathy group (HU-MSCs+DKD group). The expression of uromodulin (UMOD) and related factors in renal tissue and urine of diabetic nephropathy rats before and after HU-MSCs treatment was detected. Results: In the early stage of renal injury in DKD group, the level of UMOD in urine and renal interstitium increased (P＜0.001), and the expression of distal tubule marker protein SLC12a3 decreased. Compared with the control group, HU-MSCs treatment significantly reduced serum urea nitrogen (P＜0.01), 24-hour urinary protein (P＜0.05), and urinary albumin / creatinine ratio (P＜0.05) in DKD rats. After HU-MSCs treatment, glomerular volume decreased, mesangial cells and basement membrane thickening decreased, renal tubular injury decreased, urine and renal interstitial UMOD expression decreased, and SLC12a3 level increased. Conclusion: In the early stage of diabetic nephropathy, HU-MSCs can reduce renal tissue injury, and its reduction of renal tubular injury is related to the decrease of uromodulin in renal tissue.
ZHAO Junhong
,
MI Yan
,
WANG Caili
,
LYU Li
. Effect of human umbilical cord mesenchymal stem cells on the expression of uromodulin in kidney tissues of rats with diabetic nephropathy[J]. Journal of Baotou Medical College, 2025
, 41(5)
: 37
-41
.
DOI: 10.16833/j.cnki.jbmc.2025.05.007
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