Objective: To observe the effects of ribokinase (Rbks) gene knockout on phenotype and glucose and lipid metabolism in C57BL/6J mice. Methods: 12 male 8-week-old wide type (WT) and Rbks gene knockout (KO) C57BL/6J mice were fed for 10 weeks. The changes of appearance, body weight, food intake, water intake and fasting blood glucose were observed regularly. At the end of the observation period, the contents of triglyceride and cholesterol were measured. Results: After being fed normally for 10 weeks, compared with WT mice, KO mice had no significant difference in appearance and body weight(P＞0.05), but food intake and water intake decreased, fasting blood glucose level increased significantly(P＜0.05), serum triglyceride content increased and total cholesterol content decreased (all P＜0.05). Conclusion: Although Rbks gene knockout has no effect on the shape and weight of mice, it will affect the balance of glucose and lipid metabolism in mice.
YANG Zhongbin
,
GUO Yajing
,
WANG Zeying
,
CHEN Ziru
,
LI Houyu
,
HE Qi
,
LIU Jiahui
,
MA Shibo
,
JU Hongge
,
LI Xiaojing
,
LIU Zhi
,
SU Yan
. Effects of Rbks gene knockout on glucose and lipid metabolism in C57 mice[J]. Journal of Baotou Medical College, 2025
, 41(5)
: 33
-36
.
DOI: 10.16833/j.cnki.jbmc.2025.05.006
[1] 赫荣乔. 核糖代谢失调在糖尿病脑病中的作用[C]. 中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年论文集. 太原, 2019: 42.
[2] Teslaa T, Ralser M, Fan J, et al. The pentose phosphate pathway in health and disease[J]. Nat Metab, 2023, 5(8): 1275-1289.
[3] Bork P, Sander C, Valencia A. Convergent evolution of similar enzymatic function on different protein folds: the hexokinase, ribokinase, and galactokinase families of sugar kinases[J]. Protein Sci, 1993, 2(1): 31-40.
[4] Tai Y, Zhang ZH, Liu Z, et al. D-ribose metabolic disorder and diabetes mellitus[J]. Mol Biol Rep, 2024, 51(1): 220.
[5] 刘瑞芳. 高脂饲料诱导的胰岛素抵抗小鼠肾脏损伤及能量代谢特征的研究[D]. 哈尔滨:东北农业大学, 2023.
[6] García-Domínguez E, Carretero A, Viña-Almunia A, et al. Glucose 6-P dehydrogenase-an antioxidant enzyme with regulatory functions in skeletal muscle during exercise[J]. Cells, 2022, 11(19): 3041.
[7] Zimmer HG, Ibel H, Suchner U, et al. Ribose intervention in the cardiac pentose phosphate pathway is not species-specific[J]. Science, 1984, 223(4637): 712-714.
[8] Norton L, Shannon C, Gastaldelli A, et al. Insulin: The master regulator of glucose metabolism[J]. Metabolism, 2022, 129: 155142.
[9] Van Gerwen J, Shun-Shion AS, Fazakerley DJ. Insulin signalling and GLUT4 trafficking in insulin resistance[J]. Biochem Soc Trans, 2023, 51(3): 1057-1069.
[10] Luo J, Yang HY, Song BL. Mechanisms and regulation of cholesterol homeostasis[J]. Nat Rev Mol Cell Biol, 2020, 21(4): 225-245.
[11] 赵智博, 满振涛, 李伟. 胆固醇代谢在骨关节炎疾病中的作用及研究进展[J]. 山东大学学报(医学版), 2024, 62(2): 1-9.
