Objective: To investigate the expression of miR-27a-3p in chronic intermittent hypoxia (CIH) and its involvement in insulin resistance through biological analysis. Methods: Download the miRNA expression dataset under CIH conditions from GEO, use the EdgeR package of R software for differential miRNA screening, and select differential miR-27a-3p as the research object. Using Target Scan, miRRisk, and miRDB to predict the target genes of miR-27a-3p and obtain the intersection for GO and KEGG enrichment analysis. The gene set of insulin resistance was retrieved through genecard, and the intersection of miR-27a-3p target genes was taken. Then, a PPI network of miR-27a-3p target genes was constructed, central genes were screened, and a miRNA mRNA pathway regulatory network was constructed. Results: a total of 37 differentially expressed miRNAs were screened from the GSE210021 dataset, among which miR-27a-3p was one of the down regulated genes. There are 505 intersecting target genes of miR-27a-3p in three databases, mainly involved in the regulation of protein serine/threonine kinase activity and other biological processes, as well as the MAPK signaling pathway. There are 137 intersecting target genes between the insulin resistance gene set and miR-27a-3p, including central genes such as EGFR. The miRNA mRNA pathway shows that miR-27a-3p is a key regulatory factor in the MAPK signaling pathway and other pathways. Conclusion: Under chronic intermittent hypoxia, miR-27a-3p expression is downregulated, which may be involved in the occurrence of insulin resistance by acting on target genes such as MAPK14. The mechanism may involve related signaling pathways of MAPK and other targets.
周雪利魏翠英
. A study on the bioinformatics analysis of miR-27a-3pexpression in chronic intermittent hypoxia and its mechanism involved in insulin resistance[J]. Journal of Baotou Medical College, 2025
, 41(5)
: 26
-32
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DOI: 10.16833/j.cnki.jbmc.2025.05.005
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