Objective: To observe the effect of fire needle on the cartilage morphology of knee joint in rats with knee osteoarthritis (KOA), and to explore the mechanism of fire needle in the treatment of KOA. Methods: Twenty-one SD rats were divided into model group (n=13) and control group (n=8). The model group was injected with 0.2 mL of 4% papain into the knee joint cavity. Three rats were randomly selected from each group for modeling identification. The remaining 10 rats in the model group were randomly divided into model group and fire needle group, with 5 rats in each group. The fire needle group was treated with fire needle once every other day for 2 weeks. The model group was not treated. After intervention, the cartilage of rat knee joint was observed by naked eye, and the morphology of rat knee joint cartilage was observed by hematoxylin-eosin staining and safranin-fast green staining. Results: After modeling, the cartilage surface of the model group was rougher than that of the control group, and the chondrocytes were exposed, disordered and clustered compared with the control group. The cartilage structure was disordered compared with the control group, and the structure was not clear. After treatment, the cartilage surface of the control group was smooth and shiny; the cartilage surface of the model group was rough and lack of luster; the cartilage surface of the fire needle group was slightly rough, and the glossiness was more obvious than that of the model group. The results of hematoxylin-eosin staining and safranin O-fast green staining showed that the cartilage surface of the control group was flat, the extracellular matrix of the cartilage was evenly stained, the structure was clear, and the tidal line was intact. The model group showed rough surface, exposed chondrocytes, disordered arrangement, tidal line distortion and other pathological changes. The pathological changes of the fire needle group were lighter. Conclusion: Fire needle therapy can alleviate the knee cartilage injury and effectively treat knee osteoarthritis in rats.
WAN Tian
,
LAN Pingying
,
SHI Xinyue
. Effect of fire needle on cartilage histomorphology in rats with knee osteoarthritis[J]. Journal of Baotou Medical College, 2025
, 41(5)
: 22
-25
.
DOI: 10.16833/j.cnki.jbmc.2025.05.004
[1] Hawker GA, Croxford R, BiermanAS, et al. All-cause mortality and serious cardiovascular events in people with hip and knee osteoarthritis: a population based cohort study[J]. PLoS One, 2014, 9(3): e91286.
[2] Zeng C, Bennell K, Yang ZD, et al. Risk of venous thromboembolism in knee, hip and hand osteoarthritis: a general population-based cohort study[J]. Ann Rheum Dis, 2020, 79(12): 1616-1624.
[3] 郝聪, 李潇骁, 贺新宁, 等. 中国中老年人群症状性膝骨关节炎与髋部骨折的关系[J]. 中国现代医学杂志, 2020, 30(24): 95-100.
[4] Liu Q, Niu JB, Li H, et al. Knee symptomatic osteoarthritis, walking disability, NSAIDs use and all-cause mortality: population-based Wuchuan osteoarthritis study[J]. Sci Rep, 2017, 7: 3309.
[5] Wang YL, Nguyen UDT, Lane NE, et al. Knee osteoarthritis, potential mediators, and risk of all-cause mortality: data from the osteoarthritis initiative[J]. Arthritis Care Res, 2021, 73(4): 566-573.
[6] 中华医学会骨科学分会关节外科学组,中国医师协会骨科医师分会骨关节炎学组,国家老年疾病临床医学研究中心(湘雅医院),等.中国骨关节炎诊疗指南(2021年版)[J]. 中华骨科杂志, 2021, 41(18): 1291-1314.
[7] 洪昆达, 万甜. 毫火针治疗膝骨性关节炎30例临床观察[J]. 福建中医药, 2013, 44(6): 34-35.
[8] 郑美, 洪昆达, 陈水金, 等. 毫火针治疗膝骨性关节炎疗效观察[J]. 中国中医药现代远程教育, 2016, 14(5): 103-106.
[9] 谢川, 谢卫娜. 毫火针治疗早中期膝骨关节炎的临床疗效观察[J]. 中医临床研究, 2020, 12(23): 90-93.
[10] 王兵, 胡静, 张宁, 等. 火针扬刺治疗膝骨关节炎临床观察[J]. 中国针灸, 2017, 37(5): 463-466, 476.
[11] 李彬, 谢新才, 王麟鹏. 火针治疗膝骨关节炎临床观察[J]. 北京中医药, 2011, 30(12): 923-925.
[12] 张静, 程连顺. 毫火针留针法治疗膝骨关节炎疼痛急性发作加重的临床观察[J]. 中国中医急症, 2022, 31(2): 281-284.
[13] 宋卫平. 毫火针治疗阳虚寒凝型膝骨关节炎的临床观察[J]. 云南中医中药杂志, 2020, 41(6): 64-66.
[14] 张慧芳. 火针独穴疗法治疗早期膝骨关节炎的临床研究[J]. 中西医结合心血管病电子杂志, 2020, 8(5): 157.
[15] 张书彪. 火针扬刺法对膝骨关节炎患者膝关节疼痛程度、关节功能障碍、膝关节功能的影响研究[J]. 亚太传统医药, 2020, 16(2): 111-114.
[16] 吴明霞, 李西海, 李俐, 等. 电针对骨性关节炎软骨细胞JAK-STAT信号通路表达的影响[J]. 福建中医药大学学报, 2011, 21(6): 21-23.
[17] 王乙涵. 基于数据挖掘探析火针治疗膝骨关节炎的应用规律[D]. 北京: 北京中医药大学, 2021: 37-38.
[18] Kolasinski SL,Neogi T,Hochberg MC,et al. 2019 American college of rheumatology/arthritis foundation guideline for the management of osteoarthritis of the hand,hip,and knee[J]. Arthritis Care Res (Hoboken), 2020, 72(2): 149-162.
[19] 袁佳梦. 火针对兔膝骨关节炎血清中MMP-3、MMP-13及关节软骨中β-catenin表达的影响[D]. 呼和浩特: 内蒙古医科大学, 2020.
