Objective:To investigate the regulatory effect of hypoxia inducible factor-1α (HIF-1α) on macrophages in the thymus tissue of macrophage cell line RAW264.7 and collagen induced arthritis (CIA) mice. Methods: (1) RAW264.7 macrophages were divided into blank group, negative control group, HIF-1α-siRNA group and overexpression HIF-1α group. RT-PCR and Western blot (WB) were used to detect the expression of HIF-1α at mRNA and protein levels. The relative expression of CD206 and Arg in the four groups of cells was detected by RT-PCR. The proportion of CD16/32+ and CD206+ macrophages was detected by flow cytometry. (2) CIA mouse model was established by bovine type Ⅱ collagen in DBA/1 mice. The experiment was divided into blank group, model group, negative control group, HIF-1α-siRNA group and overexpressed HIF-1α group. The proportion of F4/80+CD16/32+M1 and F4/80+CD206+M2 macrophages in mouse thymus was detected by flow cytometry. HE staining was used to observe the pathological changes of ankle joint in mice. Results: (1)Silencing HIF-1α could decrease the proportion of F4/80+CD16/32+M1 macrophages in mouse thymus tissue, and increase the proportion of F4/80+CD206+M2 macrophages in RAW264.7 cell line and mouse thymus tissue. Overexpression of HIF-1α had the opposite effect. (2)The synovium of the ankle joint of CIA mice was proliferated, with a large number of cell infiltration. While, HIF-1α-siRNA could reduce the infiltration of lymphocytes in the synovial tissue and reduce the proliferation of the synovial membrane. Overexpression of HIF-1α has the opposite effect. Conclusion: HIF-1α can regulate the differentiation of macrophages in RAW264.7 cell line and the transformation of M1 and M2 macrophages in thymus tissue of CIA mice, thus affecting the progress of joint inflammation in CIA mice, indicating that HIF-1α can mediate the disease process of CIA mice by regulating the polarization of macrophages.
GAO Peiyuan
,
PANG Chunyan
,
ZHANG Wenlan
. Effect of HIF-1α on thymic macrophages in CIA mice and its regulation on RAW264.7 macrophages[J]. Journal of Baotou Medical College, 2025
, 41(4)
: 23
-28
.
DOI: 10.16833/j.cnki.jbmc.2025.04.005
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