Objective: To study the expression of lncRNA SNHG10 and methyltransferase METTL3 in colorectal cancer (CRC) tissues and adjacent tissues. Meanwhile, to explore the potential relationship between lncRNA SNHG10 and METTL3 in CRC. Methods: Tumor tissue and paracancerous tissue samples of 15 CRC patients were collected. The expression of lncRNA SNHG10 and METTL3 in CRC was investigated by bioinformatics, real-time fluorescence quantitative PCR (RT-qPCR) and Western blot. The relationship between the relative expression level of lncRNA SNHG10 in colorectal cancer and prognosis was analyzed. The gene interaction was analyzed by TCGA database, ENCORI, cBioPortal website and in vitro inhibition experiment of colon cancer cell line (HCT116). Results: Bioinformatics website and RT-qPCR experiments showed that lncRNA SNHG10 was up-regulated in colorectal cancer compared with adjacent tissues (P＜0.05). The high expression of lncRNA SNHG10 indicated that CRC patients were more likely to have lymph node metastasis, and the disease-specific survival rate and overall survival rate were relatively low (P＜0.05). RT-qPCR and Western blot results confirmed that METTL3 was highly expressed in CRC tumor tissues. In vitro experiments showed that knockdown of lncRNA SNHG10 in HCT116 cells resulted in down-regulation of METTL3 expression at both mRNA and protein levels (P＜0.05). Conclusion: The high expression of lncRNA SNHG10 is associated with tumor progression and poor prognosis. LncRNA SNHG10 is positively correlated with METTL3 and regulates METTL3 expression. This study suggests that SNHG10 may be a promising CRC biomarker and therapeutic target.
ZHAO Shengjie
,
LI Ruibin
,
WAN Zhiheng
. Correlation between LncRNA SNHG10 and METTL3 in colorectal cancer[J]. Journal of Baotou Medical College, 2025
, 41(3)
: 41
-47
.
DOI: 10.16833/j.cnki.jbmc.2025.03.008
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