Objective: To investigate the effect of berberine (BBR),a traditional Chinese medicine extract,on doxorubicin (DOX) induced myocardial cell damage at the cellular level,and explore the possible mechanisms involved in this process. Methods: H9C2 rat cardiomyocytes were cultured,and DOX was divided into different concentrations to determine the concentration of DOX.Then they were divided into normal control group,DOX model group and treatment group (low-dose BBR,medium-dose BBR,high-dose BBR) to treat H9C2 cells. The cell growth trend and cell morphology were observed by inverted microscope. The cell viability was determined by cell counting kit (CCK8) method. The apoptosis was detected by flow cytometry. The relative expression levels of apoptosis and autophagy related proteins Bax,Bcl-2,Beclin1 and P62 in different groups were determined by Western-blot. Results: Under the microscope,it was found that with the increase of DOX concentration,the degree of myocardial cell injury increased in turn. CCK8 method was used to select 1 μmol/L DOX as the optimal modeling concentration for myocardial injury,compared with the control group,different concentrations of BBR in the treatment group could increase the viability of DOX-induced myocardial injury cells. The expression of pro-apoptotic protein Bax and autophagy-related protein Beclin1 in the middle dose of the treatment group was significantly decreased,and the expression of anti-apoptotic protein Bcl-2 and autophagy-related protein P62 was increased (P＜0.05). Conclusion: Berberine can protect DOX-induced cardiomyocyte injury by reducing apoptosis,and this protective mechanism is related to autophagy.
SU Rina
,
HAN Xuanmao
. Study on the role of berberine in doxorubicin induced myocardial cell injury[J]. Journal of Baotou Medical College, 2025
, 41(3)
: 36
-40
.
DOI: 10.16833/j.cnki.jbmc.2025.03.007
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