Triptolide affects the progression of rheumatoid arthritis by interfering with neutrophils

  • LIU Pengyuan ,
  • SANG Yali ,
  • BAI Li ,
  • WANG Yongfu
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  • The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014010, China

Received date: 2024-07-22

  Online published: 2025-03-26

Abstract

Objective: To explore whether triptolide can alleviate the disease by reducing the infiltration of neutrophils in the joint cavity of collagen-induced arthritis (CIA) mice, and to analyze whether triptolide can reduce the formation of neutrophil extracellular capture networks (NETs) in patients with rheumatoid arthritis (RA) and its mechanism of action, so as to provide theoretical support for the clinical application of tripterygium glycosides in RA. Methods: The core targets of Tripterygium wilfordii intervention in RA disease were explored by using the public database of network medicine. After the CIA model was constructed using DBA1 mice, triptolide was given for 60 days. The hind limbs of the mice were collected for HE staining and the degree of inflammatory cell infiltration in the joint cavity of the mice was observed. Neutrophils in peripheral blood of RA patients were collected and stimulated with triptolide for 2 h. The formation of NETs was observed by immunofluorescence staining. Treatment with triptolide in RA group and triptolide could alleviate joint swelling in CIA mice; triptolide could also inhibit the formation of NETs in peripheral blood neutrophils of RA patients. Transcriptome sequencing was performed on neutrophils in RA group and Tripterygium wilfordii treatment group. Results: Through network pharmacology analysis, PPI protein interaction was performed on the locked overlapping genes, and KEGG and GO analysis were performed. It was found that IL-17, TNF signaling pathway and apoptosis-related pathway were closely related to RA disease. In vivo experiments showed that 389 differential genes were screened from the transcriptome sequencing results of the triptolide treatment group. The GO and KEGG pathways were mainly enriched in JAK-STAT, TNF, and PI3K-Akt signaling pathways. Conclusion: Triptolide can alleviate joint swelling in CIA mice by down-regulating neutrophil infiltration in the joint cavity of CIA mice, and triptolide can reduce the formation of NETs in peripheral blood of RA.This phenomenon may be related to JAK-STAT signaling pathway, TNF signaling pathway, PI3K-Akt and other signaling pathways.

Cite this article

LIU Pengyuan , SANG Yali , BAI Li , WANG Yongfu . Triptolide affects the progression of rheumatoid arthritis by interfering with neutrophils[J]. Journal of Baotou Medical College, 2025 , 41(3) : 30 -35 . DOI: 10.16833/j.cnki.jbmc.2025.03.006

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