Objective: To study and analyze the changes of intestinal flora in 1-5 years after cholecystectomy. Methods: A total of 15 fecal samples of healthy people and 16 fecal samples of people 1 to 5 years after cholecystectomy were collected from Baotou Central Hospital. The collected samples were sequenced to analyze the composition, richness and diversity of intestinal microbial flora in the two groups, and to compare the differences of intestinal microbial flora between the two groups. Results: (1) At the phylum level, the relative abundance of Bacteroidetes, Proteobacteria, Verrucomicrobia and Fusobacteria increased, while the relative abundance of Actinobacteria decreased in the population 1-5 years after cholecystectomy. (2) At the genus level, the relative abundance of Escherichia, Ruminococcus, Blautia, and Gemmatiaceae increased, while the relative abundance of Faecalibacterium, Bifidobacterium, Collins, and Agathobacter decreased in the population 1 to 5 years after cholecystectomy. The Chao1 index and Observed _ species index of the healthy population group were higher than those of the population group 1-5 years after cholecystectomy, and the differences were statistically significant (P＜0.05). The Pielou evenness of the healthy population group was slightly lower than that of the population group 1-5 years after cholecystectomy, and the difference was statistically significant (P＜0.05). Lachnospiraceae, Ruminococcus, Faecalimonas and Burkholderia were the intestinal flora with significant differences between the groups in the population group from 1 to 5 years after cholecystectomy. Conclusion: (1) The composition of intestinal flora in the population 1-5 years after cholecystectomy has changed. (2) The relative abundance of short-chain fatty acid-producing bacteria and probiotics in the population 1-5 years after cholecystectomy decreased, while the relative abundance of pathogenic bacteria increased.
DONG Zhenyu
,
DU Mingyue
,
GUO Jing
,
HOU Runjie
,
WANG Jijun
. Analysis of changes of human intestinal flora after cholecystectomy[J]. Journal of Baotou Medical College, 2025
, 41(3)
: 6
-12
.
DOI: 10.16833/j.cnki.jbmc.2025.03.002
[1] Qi L, Tian Y, Chen YS. Gall bladder: The metabolic orchestrator[J]. Diabetes Metab Res Rev, 2019,35(5):e3140.
[2] 李瑞,游海波. 胆囊切除术后腹泻的研究进展[J]. 检验医学与临床,2023,20(22):3389-3392,3415.
[3] 刘欢欢,田雨,彭洋,等. 胆囊切除术后综合征的诊治和预防[J]. 临床肝胆病杂志,2018,34(11):2464-2468.
[4] Dong ZY, Shi RX, Li PD, et al. Does postcholecystectomy increase the risk of colorectal cancer[J]. Front Microbiol, 2023,14:1194419.
[5] Saraste D, Jaras J, Martling A. Population-based analysis of outcomes with early-age colorectal cancer[J]. Br J Surg, 2020,107(3):301-309.
[6] Cho YA, Lee J, Oh JH, et al. Genetic risk score, combined lifestyle factors and risk of colorectal cancer[J]. Cancer Res Treat, 2019,51(3):1033-1040.
[7] Bundgaard-Nielsen C, Baandrup UT, Nielsen LP, et al. The presence of bacteria varies between colorectal adenocarcinomas, precursor lesions and non-malignant tissue[J]. BMC Cancer, 2019,19(1):399.
[8] ábraham S, Nemeth T, Benko R, et al. Evaluating the distribution of the locations of colorectal cancer after appendectomy and cholecystectomy[J]. World J Surg Oncol, 2020,18(1):94.
[9] Ren XH, Xu J, Zhang YY, et al. Bacterial alterations in post-cholecystectomy patients are associated with colorectal cancer[J]. Front Oncol, 2020,10:1418.
[10] Chen CH, Lin CL, Kao CH. The effect of cholecystectomy on the risk of colorectal cancer in patients with gallbladder stones[J]. Cancers (Basel), 2020,12(3):550.
[11] Lee J, Choe S, Park JW, et al. The risk of colorectal cancer after cholecystectomy or appendectomy: a population-based cohort study in Korea[J]. Yebang Uihakhoe Chi, 2018,51(6):281-288.
[12] Jiang X, Jiang ZX, Cheng Q, et al. Cholecystectomy promotes the development of colorectal cancer by the alternation of bile acid metabolism and the gut microbiota[J]. Front Med, 2022,9:1000563.
[13] Ma YP, Qu RZ, Zhang Y, et al. Progress in the study of colorectal cancer caused by altered gut microbiota after cholecystectomy[J]. Front Endocrinol (Lausanne), 2022,13:815999.
[14] 翟晓,杨芸,王文学,等. 胆囊切除术与结直肠癌发病的关系[J]. 中华消化杂志,2019,39(10):715-717.
[15] Cao HL, Xu MQ, Dong WX, et al. Secondary bile acid-induced dysbiosis promotes intestinal carcinogenesis[J]. Int J Cancer, 2017,140(11):2545-2556.
[16] 陈功雪. 肠道调节性T细胞组成及功能[J]. 中国医药科学,2019,9(12):29-31.
[17] 王林,周锋,闫昌盛,等. 胆囊切除术后肠道菌群失调的研究进展[J]. 中华消化外科杂志,2018,17(3):321-324.
[18] 张伟鹏,孙逸飞,田忠. 胆囊切除与结直肠癌的关系研究进展[J]. 肝胆胰外科杂志,2021,33(1):53-55,60.
[19] Valguarnera E, Wardenburg JB. Good gone bad: one toxin away from disease for Bacteroides fragilis[J]. J Mol Biol, 2020,432(4):765-785.
[20] 郑冰峰,陈泽旭,郑琦,等. 胆囊切除术后胆汁酸代谢及其诱发结直肠癌机制的研究进展[J]. 中华消化外科杂志,2020,19(5):559-562.
[21] Peng C, Ouyang YB, Lu NH, et al. The NF-κB signaling pathway, the microbiota, and gastrointestinal tumorigenesis: recent advances[J]. Front Immunol, 2020,11:1387.
