Objective: To investigate the relationship between the activity and gene polymorphism of coagulation factor Ⅻ and the risk of recurrent spontaneous abortion (RSA) in Baotou Han population. Methods: RSA patients and healthy pregnant women were selected as the research objects. The activity of coagulation factor Ⅻ was measured. The genotype composition of FⅫ gene single nucleotide polymorphism (SNP) rs1801020 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results of the two groups were analyzed to determine the correlation between FⅫ gene SNP rs1801020 and RSA under different genetic models. Results: There were significant differences in the genotype and allele frequency distribution of SNP rs1801020 in FⅫ gene between the two groups (P＜0.05); the frequency of T allele in RSA group was lower than that in control group (χ2=8.756, OR=2.043, 95%CI: 1.267-3.295,P<0.05); the frequency of CT genotype in RSA group was higher than that in control group, while the frequency of TT genotype was lower than that in control group, and the difference was statistically significant (χ2=9.905, OR=2.611, 95%CI: 1.447-4.713,P＜0.05); FⅫ activity in RSA patients was different among rs1801020 genotypes, and the order of activity was CC, CT and TT. FⅫ SNP rs1801020 was associated with the risk of RSA under codominant, dominant and overdominant models (OR=2.611, 2.636, 2.470, P＜0.05); compared with TT genotype, CT and CT+CC genotypes could increase the risk of RSA; compared with CC+TT genotype, CT genotype could increase the risk of RSA. Conclusion: The difference in the distribution of FⅫ rs1801020 between the two groups may be related to the risk of RSA in Baotou Han women. Compared with TT genotype, mutant heterozygous CT genotype may increase the risk of RSA, the T allele of rs1801020 locus of FⅫ gene can cause the decrease of FⅫ activity.
ZHAO Pingping
,
WU Di
,
LI Dechun
,
WANG Yanguo
. Correlation analysis of FⅫ activity and rs1801020 polymorphism with recurrent spontaneous abortion in Baotou Han population[J]. Journal of Baotou Medical College, 2025
, 41(3)
: 1
-5
.
DOI: 10.16833/j.cnki.jbmc.2025.03.001
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