Objective: To study the mechanism of acute toxic target organs and pathological damage of nano-realgar drugs in Kunming mice, and to evaluate its safety. Methods: Thirty-two mice were randomly divided into control group and nano-realgar experimental 3 days group, 7 days group and 14 days group, with 8 mice in each group. The control group was given normal saline by gavage, and the experimental group was given nano-realgar suspension 5 000 mg/kg by gavage, once a day. The mice in the experimental group were sacrificed on the 3rd, 7th and 14 th day after administration, and the mice in the control group were sacrificed on the 14th day. The general condition, body weight change, blood biochemical index determination and pathological evaluation were observed. Results: After administration, there was no significant difference in general condition and body weight between the control group and the experimental group. Compared with the control group, the albumin, albumin-to-globulin ratio and urea nitrogen in the experimental group were decreased (P＜0.05), the total cholesterol in the experimental 7 days group was increased (P＜0.05), and the alkaline phosphatase in the experimental 14 days group was increased (P＜0.01). Pathological observation showed that the cytoplasm of hepatocytes in the experimental group was loose, and a small number of translucent vacuoles of different sizes appeared in the cytoplasm; the alveolar structure was slightly damaged and the alveolar septum was widened; the boundary between red pulp and white pulp of the spleen was slightly unclear, and significant megakaryocytes appeared in the red pulp area. Conclusion: There is no obvious acute toxicity when mice are given by gavage at 5 000 mg/kg, which proves that the safety of nanorealgar is good.
LI Yuanyuan
,
MA Qiang
,
JU Hongge
. Study on acute toxicity evaluation and pathological damage mechanism of nano-realgar[J]. Journal of Baotou Medical College, 2025
, 41(2)
: 47
-51
.
DOI: 10.16833/j.cnki.jbmc.2025.02.009
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