Objective: To explore the neurobiological mechanism of acupuncture combined with medicine in the treatment of cerebral ischemia by observing the synergistic effect of gastrodin combining with electro-acupuncture (EA) on the expression of growth associated protein 43(GAP-43) and synaptophysin (SYP) in caudate putamen nucleus of rats with focal cerebral ischemia (FCI). Methods: A total of 48 Sprague-Dawley (SD) rats were randomly divided into 6 groups (normal group, sham operation group, model group, gastrodin group, EA group and gastrodin+EA group). The model was prepared by middle cerebral artery occlusion (MCAO). 2 weeks after modeling, rats were given gastrodin, EA, or gastrodin+EA for continuous treatment in 2 successive weeks. Then expressions of GAP-43 and SYP protein in the caudate putamen nucleus were detected by immunohistochemistry. Results: Positive expression of GAP-43 and SYP protein was found in each group of rats. Expressions of GAP-43 in model group was higher than the normal group and sham operation group (P＜0.05), expressions of SYP in model group was lower than the normal group and sham operation group (P＜0.05). Compared with the model group, the positive expressions of GAP-43 and SYP in gastrodin group, the EA group, and the gastrodin+EA group increased significantly (P＜0.05). The positive expressions of GAP-43 and SYP increased obviously in the gastrodin+EA group compared to those of the EA group and the medication group (P＜0.05). Conclusion: Gastrodin administration and EA intervention can up-regulated the expressions of GAP-43 and SYP protein in the caudate putamen nucleus of FCI rats, which may contribute action in promoting the repair of injured neurons. The effect of gastrodin+EA is better than simple gastrodin or EA treatment.
LI Chengyu
,
NIE Zeyin
,
MIAO Huachun
,
WU Feng
. Effect of gastrodin and electro-acupuncture on expression of growth-associated protein 43 and synaptophysin in caudate putamen of focal cerebral ischemia rats[J]. Journal of Baotou Medical College, 2025
, 41(2)
: 35
-38
.
DOI: 10.16833/j.cnki.jbmc.2025.02.007
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