Objective: To explore the influencing factors of pomegranate seed oil and fish oil on the expression of IL-1βmRNA in knee cartilage in rats with osteoarthritis (OA), and to compare the effects of pomegranate seed oil and fish oil on knee arthritis (KOA), so as to expand new ideas and provide scientific basis for the prevention and treatment of KOA. Methods: A total of 154 8-week-old healthy female SD rats were selected, and the experimental animals were randomly divided into 7 groups by EXCEL: Sham group, Model group, normal saline (NS) group, pomegranate seed oil low-dose (PSOLD) group, pomegranate seed oil high-dose (PSOHD) group, fish oil low-dose (FOLD) group, and fish oil high-dose (FOHD) group, with 22 rats in each group. The modified Hulth method was used to establish the rat KOA model, and the representative two omega-3 polyunsaturated fatty acids (n-3PUFAs), pomegranate seed oil (PSO) and fish oil (FO) experimental groups were selected for intervention, and the Sham group and the Model group did not intervene, the rats in the NS group were given 1 ml/kg of normal saline per day, and the PSOLD group and PSOHD group were given 0.83 ml/kg and 1.66 ml/kg PSO gavage, respectively. The FOHD group was given 0.69 ml/kg and 1.38 ml/kg by gavage, respectively. After 8 weeks of intervention, 10 rats in each group were randomly sacrificed, and the cartilage specimens of the knee joint of the hind limbs of the rats were taken for RT-PCR. After 12 weeks of intervention, the remaining 10 rats in each group were sacrificed, and the treatment was the same as above. Results: Compared with the Sham group, the relative expression of IL-1β mRNA in the Model group and the experimental groups was significantly increased at 8 weeks and 12 weeks (P＜0.01). Compared with the Model group, the relative expression of IL-1β mRNA in the PSO and FO groups was significantly decreased at 8 and 12 weeks (P＜0.01). There was no significant difference among the other groups (P＞0.05). The relative expression of IL-1β mRNA in Model group and NS group increased significantly at 8 weeks and 12 weeks (P＜0.05), while there was no significant difference between the other groups (P＞0.05). Conclusion: (1) Pomegranate seed oil and fish oil can effectively reduce the relative expression of IL-1βmRNA and improve cartilage regression. (2) There is no significant difference in the effect of down-regulating IL-1βmRNA expression between the PSOLD group and the PSOHD group, the FOLD group and the FOHD group with the same intervention duration. (3) From the analysis of 8-week and 12-week data, there is no significant correlation between the expression of IL-1β mRNA and the length of intervention in the two groups of PSO and FO groups.
LYU Tenghui
,
ZHANG Tao
,
XING Jialu
,
LI Rui
,
QI Hedong
,
CHEN Zhouyang
,
SHI Qingtian
,
MA Zhipeng
,
LI Yuan
,
XU Zhixiong
,
XU Wensheng
. Effects of pomegranate seed oil and fish oil on IL-1β-mediated cartilage degeneration in knee osteoarthritis[J]. Journal of Baotou Medical College, 2025
, 41(2)
: 18
-23
.
DOI: 10.16833/j.cnki.jbmc.2025.02.004
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