Objective: To evaluate the safety of the ethyl acetate fraction of Panzerina alaschanica and investigate its anti-inflammatory effects using a zebrafish inflammation model. Methods: Wild-type zebrafish was used to study the effects of different concentrations of Panzerina alaschanica on embryo mortality, deformity rate and body length, and its safety was evaluated. Transgenic zebrafish was used to observe the migration of green fluorescent protein-labeled macrophages after treatment with different concentrations of Panzerina alaschanica for 6 h, and its anti-inflammatory activity was evaluated. Results: Low concentrations of Panzerina alaschanica had no significant effect on the development of zebrafish embryos after short-term and long-term effects, but with the prolongation of concentration and time, the drug would have a negative impact on its development. Compared with the blank control group, the number of macrophage migration in the low, medium and high concentrations of Panzerina alaschanica treatment group was significantly reduced, indicating that the ethyl acetate fraction of Panzerina alaschanica could significantly inhibit the migration of zebrafish macrophages to the inflammatory site, showing a significant anti-inflammatory effect. Conclusion: This study provides direct evidence of the anti-inflammatory effects of Panzerina alaschanica through the observation of GFP-labeled macrophage migration. However, with the increase of the concentration, the Panzerina alaschanica has a serious negative impact on the development of zebrafish. This suggests that in clinical applications, the dose and duration of use of Panzerina alaschanica need to be strictly controlled to avoid possible toxicity and side effects.
WANG Lijuan
,
WANG Jinjin
,
XU Liang
,
ZHANG Na
,
LIN Yufeng
,
JIA Xiao’e
,
YANG Yanfang
. Safety evaluation and anti-inflammatory activity of ethyl acetate fraction of mongolian medicine Panzerina alaschanica[J]. Journal of Baotou Medical College, 2025
, 41(2)
: 13
-17
.
DOI: 10.16833/j.cnki.jbmc.2025.02.003
[1] 米盈盈. 蒙药白益母草生药学及抗炎活性研究[D]. 北京: 中央民族大学, 2021.
[2] 杨康, 阿如娜, 周雪, 等. 蒙药白益母草本草考证研究[J]. 中华中医药杂志, 2022, 37(7): 4130-4132.
[3] 杨康. 蒙药白益母草抗MRSA皮肤感染作用机制及其物质基础研究[D]. 北京: 中央民族大学, 2022.
[4] Chen YP, Chou CM, Chang TY, et al. Bridging size and charge effects of mesoporous silica nanoparticles for crossing the blood-brain barrier[J]. Front Chem, 2022, 10: 931584.
[5] 张数数, 霍仕霞, 李滨, 等. 基于斑马鱼模型的高良姜素安全性评价研究[J]. 药物评价研究, 2023, 46(9): 1919-1928.
[6] Yoganantharjah P, Gibert Y. The use of the zebrafish model to aid in drug discovery and target validation[J]. Curr Top Med Chem, 2017, 17(18): 2041-2055
[7] Williams CH, Hong CC. Zebrafish small molecule screens: taking the phenotypic plunge[J]. Comput Struct Biotechnol J, 2016, 14: 350-356.
[8] 贾小娥, 朱伟, 樊燕, 等. 斑马鱼组蛋白甲基转移酶在巨噬细胞炎症迁移中的作用研究[J]. 泰山医学院学报, 2017, 38(2): 126-130.
[9] Ye BX, Deng X, Shao LD, et al. Vibsanin B preferentially targets HSP90β, inhibits interstitial leukocyte migration, and ameliorates experimental autoimmune encephalomyelitis[J]. J Immunol, 2015, 194(9): 4489-4497.
[10] Zhang Y, Bai XT, Zhu KY, et al. In vivo interstitial migration of primitive macrophages mediated by JNK-matrix metalloproteinase 13 signaling in response to acute injury[J]. J Immunol, 2008, 181(3): 2155-2164.
[11] 《陈山文集》编辑委员会. 陈山文集[M]. 呼和浩特:内蒙古科学技术出版社, 2016.
[12] 内蒙古植物志编辑委员会. 内蒙古植物志[M]. 呼和浩特: 内蒙古人民出版社, 1982.
[13] 《全国中草药汇编》编写组. 全国中草药汇编[M]. 第2版. 北京: 人民卫生出版社, 1996.
[14] 《中华本草》编委会. 中华本草.蒙药卷[M]. 上海: 上海科学技术出版社, 2004.
[15] Zhang D, Xu T, Xu Q, et al. Expression profile of macrophage migration inhibitory factor in periodontitis[J]. Arch Oral Biol, 2021, 122: 105003.
[16] 秦帅. 活体高通量筛选抑制斑马鱼巨噬细胞迁移的中药[D]. 贵阳: 贵阳中医学院, 2011.
