Objective: To observe the effects of liraglutide on myocardial infarction area, myocardial oxidative and antioxidant levels, and the expression levels of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in myocardial tissue after myocardial ischemia/reperfusion injury (MIRI) by establishing an ischemia-reperfusion model of diabetes mellitus (DM) rats, and to explore the effect of liraglutide on MIRI in DM rats and its possible mechanism. Methods: Male SD rats were randomly divided into normal group, I/R group, liraglutide+I/R group, liraglutide+I/R+Nrf2 inhibitor group(Nrf2 inhibitor group), liraglutide+I/R+HO-1 inhibitor group(HO-1 inhibitor group), liraglutide+I/R+Nrf2 inhibitor+HO-1 inhibitor group (double inhibitor group). DM and ischemia-reperfusion models were constructed. Enzyme-linked immunosorbent assay (ELISA) was used to detect malondialdehyde (MDA) content and superoxide dismutase (SOD) content. The morphology of rat myocardial tissue was observed by hematoxylin-eosin (HE) staining. Western blotting was used to detect the expression of Nrf2/HO-1 pathway-related proteins in rat myocardial tissue. Results: Compared with the normal group, the MDA content in the I/R group was significantly increased, and the SOD content was significantly decreased. Compared with the I/R group, the myocardial infarction area of the liraglutide+I/R group was reduced. Compared with the I/R group, the myocardial tissue cells in the liraglutide+I/R group were arranged neatly, the myofilament contour was clear, and the intercellular space was tight and uniform. Compared with the normal group, the levels of Nrf2 and HO-1 protein in the myocardial tissue of the I/R group were significantly reduced, and the myocardial tissue showed obvious pathological damage. Compared with the I/R group, the serum MDA content of the rats in the liraglutide+I/R group was significantly reduced, the SOD content was increased, the levels of Nrf2 and HO-1 protein in the myocardial tissue were significantly increased, and the pathological damage of the myocardial tissue was reduced. Conclusion: Liraglutide can reduce myocardial ischemia-reperfusion injury in DM rats, the mechanism may be related to the activation of Nrf2/HO-1 signaling pathway.
YANG Hao
,
SUN Shuyan
. Liraglutide improves myocardial ischemia-reperfusion injury in diabetic rats by activating Nrf2/HO-1 signaling pathway[J]. Journal of Baotou Medical College, 2025
, 41(2)
: 6
-12
.
DOI: 10.16833/j.cnki.jbmc.2025.02.002
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