Objective: To explore the key genes and signaling pathways in the progression of pelvic organ prolapse (POP) by bioinformatics analysis. Methods: The data sets GSE12852 and GSE28600 were downloaded from the public database GEO. The differential genes were obtained by online tool GEO2R analysis, GO and KEGG analysis were performed by DAVID database, and PPI network between genes was analyzed by STRING database. The expression of KRT5, KRT19, CLDN7 and CLDN4 in uterosacral ligament tissues was detected by immunohistochemistry. Results: After the intersection of the differential genes of the two data sets, a total of 53 genes were lowly expressed in the sacral ligament of POP patients. GO and KEGG analysis showed that low expression genes were related to functions or pathways such as extracellular matrix components and intercellular connections. On the PPI network, KRT5, KRT19, CLDN7, and CLDN4 genes might play a key role in POP progression. Immunohistochemical experiments confirmed that the protein levels of KRT5, KRT19, CLDN7 and CLDN4 were down-regulated in the sacral ligament of POP. Conclusion: These microarray data and bioinformatics analyses provide a useful method for the identification of key genes and pathways associated with POP. Moreover, some crucial DEGs, such as KRT5, KRT19, CLDN7, CLDN4, potentially play an important role in the development and progression of POP.
WANG Li
,
SUN Yuan
,
DING Caiyun
,
DING Jin
. Bioinformatics analysis of uterosacral ligament gene chip in patients with pelvic organ prolapse[J]. Journal of Baotou Medical College, 2025
, 41(2)
: 1
-5
.
DOI: 10.16833/j.cnki.jbmc.2025.02.001
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