Objective: To observe the expression levels of regulatory T cells (Treg), helper T cells 17 (Th17) and cytokine interleukin 17 (IL-17) in peripheral blood of patients with type 1 diabetes mellitus (T1DM) and latent autoimmune diabetes in adults (LADA), with the purpose of exploring the role of Th17/Treg in T1DM by analyzing its correlation with islet-reactive B cells function in T1DM. Methods: A total of 78 cases of T1DM patients, LADA patients and normal healthy people who were admitted in the Endocrinology Department, the First Affiliated Hospital of Bengbu Medical College from December 2021 to June 2022 were selected and divided into the T1DM group, LADA group and normal control (NC) group. Serum IL-17 was detected by ELISA method. Proportions of Th17 and Treg cells were detected by flow cytometry, and the differences of Th17 and IL-17 expression among the three groups were compared. Results: The proportion of Treg cells in the T1DM group and LADA group was significantly lower than that in the NC group, and it was significantly lower in the T1DM group than that in the LADA group. The expression levels of Th17 and IL-17 in the T1DM group and LADA group were significantly higher than those in the NC group, but there was no significant difference between the T1DM group and LADA group. The results of Pearson correlation analysis showed that serum IL-17 and Th17 were negatively correlated with FCP and 2h-CP (IL-17: r/P=-0.273, 0.016, -0.352, 0.002), Treg was positively correlated with FCP and 2h-CP (r/P=0.494/<0.001, 0.575/<0.001). Multivariate logistic regression analysis showed that IL-17 was a risk factor to T1DM and LADA by taking T1DM, LADA, NC as dependent variables(1=T1DM,2=LADA,3=NC), Th17 was a risk factor to T1DM, and Treg was a protective factor to T1DM and LADA. Conclusion: T1DM and LADA patients have unbalanced Th 17 / Treg ratio in vivo, manifested by increased expression level of Th 17 and IL-17 and decreased expression level of Treg, it may be associated with functional impairment of islet-reactive B cells, which could lead to the occurrence and development of T1DM.
YIN Qingqing
,
YANG Lulu
,
HU Xiaolei
,
SHI Zhaoming
,
YANG Qingqing
,
SUN Weihua
. Study on the correlation between Th17/ Treg and IL-17 and pancreatic B cell function in type 1 diabetes mellitus[J]. Journal of Baotou Medical College, 2025
, 41(1)
: 91
-96
.
DOI: 10.16833/j.cnki.jbmc.2025.01.017
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