Objective: To explore the expression of m6A methylation regulatory enzymes and cerebral small vessel-disease related genes in peripheral blood mononuclear cells of patients with vasogenic white matter hyperintensity, and to provide a theoretical basis for the pathogenesis of vasogenic white matter hyperintensity. Methods: From October 2021 to October 2022, 12 patients with white matter hyperintensity and 12 healthy subjects were selected from the Department of Neurology, the First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology. Real-time fluorescence quantitative PCR (RT-qPCR) was used to analyze the mRNA expression of m6A methylation regulatory enzymes and brain small vessel-related genes. Western blot was used to detect the protein expression of METTL3 and ILF3. Results: Compared with the control group, the mRNA expression levels of METTL3, KAA1429 and ILF3 were lower(P＜0.05). Compared with the control group, the protein expression levels of METTL3 and ILF3 were lower(P＜0.05). Compared with the control group, there was no significant difference in the mRNA expression levels of METTL14, DGCR8, FTO, WTAP, RBM15, YTHDC1, ATF3 and ERBIN(P＞0.05). Conclusion: Methyltransferases METTL3 and ILF3 may be involved in the pathogenesis of vasogenic white matter hyperintensity.
MENG Chenxi
,
SUN Hongying
,
ZHANG Jia
,
MAO Jian
,
YANG Yang
,
CE Lemuge
. Expression analysis of m6A methylation regulatory enzymes in peripheral blood PBMC of patients with vasogenic white matter hyperintensity[J]. Journal of Baotou Medical College, 2025
, 41(1)
: 74
-77
.
DOI: 10.16833/j.cnki.jbmc.2025.01.013
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