Objective: To discuss the expression level and clinical significance of circRNA-SRRM2 in peripheral blood of patients with acute cerebral infarction. Methods: A total of 46 patients with acute cerebral infarction less than 7 days of onset were collected as the case group, and 48 patients without cerebral infarction were collected as the control group. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression level of circRNA-SRRM2 in peripheral blood in the case group and the control group. Spearman and pearson correlation analysis were used to discuss the correlation between the target gene and acute cerebral infarction. At the same time, the general data of the two groups were collected, and Logistic regression analysis was used to explore the independent risk factors of acute cerebral infarction. Results: The expression level of circRNA-SRRM2 in peripheral blood of patients with acute cerebral infarction was significantly higher than that of the control group (P＜0.05). Gender, age, systolic blood pressure, diastolic blood pressure, history of hypertension, smoking history, drinking history and peripheral blood circRNA-SRRM2 expression level were positively correlated with the occurrence of acute cerebral infarction (P＜0.05). Logistic regression analysis showed that the independent risk factors of acute cerebral infarction were gender, age, systolic blood pressure, diastolic blood pressure, history of hypertension, smoking history, drinking history and peripheral blood circRNA-SRRM2 (P＜0.05). The area under the ROC curve of peripheral blood circRNA-SRRM2 in the diagnosis of acute cerebral infarction was 0.683 (P＜0.002), the critical value was 1.673, the sensitivity was 0.820, and the specificity was 0.470. Conclusion: The expression of circRNA-SRRM2 is up-regulated in acute cerebral infarction, which is expected to provide some value for the early prevention of acute cerebral infarction and become a new biological marker in peripheral blood of patients with acute cerebral infarction in the future.
ZHOU Wenhui
,
LIU Dan
,
ZHANG Guangwei
. The expression level and clinical significance of circRNA-SRRM2 in peripheral blood of patients with acute cerebral infarction[J]. Journal of Baotou Medical College, 2025
, 41(1)
: 68
-73
.
DOI: 10.16833/j.cnki.jbmc.2025.01.012
[1] Tu WJ, Zhao Z, Yin P, et al. Estimated burden of stroke in China in 2020[J]. JAMA Netw Open, 2023, 6(3): e231455.
[2] Chen LL. The expanding regulatory mechanisms and cellular functions of circular RNAs[J]. Nat Rev Mol Cell Biol, 2020, 21(8): 475-490.
[3] Carninci P, Kasukawa T, Katayama S, et al. The transcriptional landscape of the mammalian genome[J]. Science, 2005, 309(5740): 1559-1563.
[4] 袁莉, 黎军宏, 黄秋霞, 等. 环状RNA及其调控网络在缺血性脑卒中的潜在机制探讨[J]. 中华老年心脑血管病杂志, 2022, 24(11): 1230-1232.
[5] Kour B, Gupta S, Singh R, et al. Interplay between circular RNA,microRNA, and human diseases[J]. Mol Genet Genomics, 2022, 297(2): 277-286.
[6] Ashwal-Fluss R, Meyer M, Pamudurti NR, et al. circRNA biogenesis competes with pre-mRNA splicing[J]. Mol Cell, 2014, 56(1): 55-66.
[7] Li Y, Zheng Q, Bao C, et al. Circular RNA is enriched and stable in exosomes: a promising biomarker for cancer diagnosis[J]. Cell Res, 2015, 25(8): 981-984.
[8] Jan AT, Malik MA, Rahman S, et al. Perspective insights of exosomes in neurodegenerative diseases:a critical appraisal[J]. Front Aging Neurosci, 2017, 9: 317.
[9] 何松照. 急性脑梗死发病危险因素分析[J]. 中国现代药物应用, 2010, 4(14): 40-41.
[10] 刘磊, 冯浩, 杨兴东, 等. 急性脑梗死发病危险因素分析[J]. 山东医药, 2020, 60(12): 3.
[11] Hu XL, Su Q, Meng DL, et al. Circular RNA expression alteration and bioinformatics analysis in patients with acute cerebral infarction injury [J]. Bioengineered, 2021, 12(2): 11490-11505.
[12] 梁雪莹, 赵直, 龙建雄, 等. 外周血hsa-circ-0105035表达水平与急性缺血性脑卒中的相关性[J]. 微创医学, 2023, 18(2): 151-158.
[13] Yang L, Han B, Zhang Z, et al. Extracellular vesicle-mediated delivery of circular RNA SCMH1 promotes functional recovery in rodent and nonhuman primate ischemic stroke models[J]. Circulation, 2020, 142(6): 556-574.
[14] Wang X, Su D, Wei Y, et al. Effect of GATAD1 regulating the SRRM2 gene on recurrence of thyroid tumor and its molecular mechanism[J]. Gland Surg, 2022, 11(12): 1897-1907.
[15] Cuinat S, Nizon M, Isidor B, et al. Loss-of-function variants in SRRM2 cause a neurodevelopmental disorder[J]. Genet Med, 2022, 24(8): 1774-1780.
[16] Fazeli S, Motovali-Bashi M, Peymani M, et al. A compound downregulation of SRRM2 and miR-27a-3p with upregulation of miR-27b-3p in PBMCs of Parkinson's patients is associated with the early stage onset of disease[J]. PLoS One, 2020, 15(11): e0240855.
[17] 唐诗, 田步先. 急性脑梗死患者的脂肪酸代谢组学相关性分析[J]. 中风与神经疾病杂志, 2021, 38(12): 1081-1084.
