Objective: To study the correlation of disease severity with the expression of inflammatory factors by analyzing the differences in expression of serum miR-142-3P in different clinical stages of patients with knee osteoarthritis (KOA). Methods: A total of 60 patients who diagnosed as KOA in the First Affiliated hospital of Baotou Medical College were selected as the observation group, they were divided into three groups according to the Kellgren-Lawrence classification and Chinese Medical Association Guidelines for the Diagnosis and Treatment of Osteoarthritis (2018 edition), which are the groups of early, middle and late stages, with 20 patients in each group. A total of 20 healthy subjects were selected as the control group. The expression levels of miR-142-3P and inflammatory factors IL-6, IL-1β and TNF-α were measured using reverse transcript-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). SPSS 23.0 Statistical software was used to analyze expression variability, and Spearman correlation analysis was used to analyze relationship between serum miR-142-3P and clinical stage as well as expression of inflammatory factors in KOA patients. Results: Compared with the control group, miRNA-142-3P expressions were increased in the early, middle and late stages of the observation group, and the differences were statistically significant (P＜0.05), and it was lower in the late stage than that in the middle stage. Compared with the control group, IL-6 expressions were increased in the early, middle and late stages of the observation group, and the differences were statistically significant (P＜0.05), and it was lower in the late stage than that in the middle stage. Compared with the control group, IL-1 β expression was increased in the early, middle and late stages of the observation group, and the differences were statistically significant (P＜0.05). Compared with the control group, TNF- α expression was increased in the early, middle and late stages, and the differences were statistically significant (P＜0.05). The miRNA-142-3P, IL-6, IL-1β and TNF-α expression levels were all positively correlated with the clinical stage of KOA (respectively, r=0.450, P=0.000;r=0.405 5, P=0.001 3;r=0.938, P＜0.000 1;r=0.943, P＜0.000 1). The expression level of miRNA-142-3P was positively correlated with IL-6 (r=0.699 0, P＜0.000 1), IL-1β(r=0.504 8, P＜0.000 1) and TNF-α (r=0.514 7, P＜0.000 1). Conclusion: miRNA-142-3p expression is positively correlated with KOA stages, as well as inflammatory cytokines IL-6, IL-1 β, and TNF- α.
MA Zhipeng
,
ZHANG Tao
,
BADE Rengui
,
SUN Jinlei
,
ZHOU Dongming
,
WANG Yulu
,
XU Wensheng
. Correlation of human serum miR-142-3p on severity of osteoarthritis and expression of inflammatory cytokines in knee osteoarthritis[J]. Journal of Baotou Medical College, 2025
, 41(1)
: 49
-53
.
DOI: 10.16833/j.cnki.jbmc.2025.01.009
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