Objective: To investigate the protective effect of berberine (BBR) on daunorubicin (DNR)-induced cardiomyocyte injury and the role of CaN-NFAT pathway. Methods: H9C2 cardiomyocytes were randomly divided into 4 groups : CON group, DNR group, DNR + BBR group and DNR + VIVIT (pathway inhibitor) group. The morphology of H9C2 cardiomyocytes was observed by microscope. CCK-8 method was used to detect the survival rate of H9C2 cardiomyocytes. The injury of H9C2 cardiomyocytes was detected by biochemical kit. Western Blot was used to detect the expression of NFATc4 protein and apoptotic proteins Bax and Bcl-2. Results: (1)Cell morphology under the microscope: compared with the CON group, the number of cardiomyocytes in the DNR group decreased. Compared with the DNR group, the number of cardiomyocytes in the DNR+BBR group and the DNR+VIVIT group increased. (2)CCK-8 assay: compared with CON group, the survival rate of cardiomyocytes in DNR group was significantly decreased(P＜0.05). Compared with DNR group, the survival rate of cardiomyocytes in DNR+BBR group and DNR+VIVIT group was increased(P＜0.05). (3)Creatine kinase (CK) content and lactate dehydrogenase (LDH) release: ①Compared with the CON group, the CK activity of the DNR group increased(P＜0.05). Compared with the DNR group, the CK content in the DNR+BBR and DNR+VIVIT groups decreased(P＜0.05). ②Compared with the CON group, the LDH release in the DNR group was increased(P＜0.05). Compared with the DNR group, the LDH release in the DNR+BBR and DNR+VIVIT groups was decreased(P＜0.05). (4)Western Blot: ①Compared with the CON group, the expression of Bax, Bax/Bcl-2 ratio and NFATc4 in the DNR group increased, while the expression of Bcl-2 decreased(P＜0.05). ②Compared with DNR group, the expression of Bax, Bax/Bcl-2 and NFATc4 in DNR+BBR group and DNR+VIVIT group decreased, while the expression of Bcl-2 increased(P＜0.05). Conclusion: BBR may inhibit DNR induced cardiomyocyte injury and reduce apoptosis by regulating CaN-NFAT signaling pathway.
PANG Hanrui
,
HAN Xuanmao
,
LIN Xuefeng
. Reducing effect of berberine on daunorubicin-induced cardiomyocyte injury by regulating CaN-NFAT signaling pathway[J]. Journal of Baotou Medical College, 2025
, 41(1)
: 43
-48
.
DOI: 10.16833/j.cnki.jbmc.2025.01.008
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