Objective: To investigate the effect and mechanism of puerarin (Pue) in the treatment of postmenopausal osteoporosis (PMOP) in rats. Methods: Female SD rats were divided into sham operation group (SHAM group), model group (PMOP group), low-dose puerarin group (Pue-L group) and high-dose puerarin group (Pue-H group). In the SHAM group, partial fat around the ovary was removed, and bilateral ovaries were removed in the other groups. Bone mineral density (BMD) and hematoxylin-eosin (HE) staining were used to observe the femoral tissue at 4 weeks after operation to determine whether the model was successful. After successful modeling, rats in Pue-L group and Pue-H group were subcutaneously injected with different concentrations of puerarin injection, and rats in SHAM group and PMOP group were injected with the same amount of normal saline. BMD of rats was detected after 8 weeks of administration. The bone structure was observed by micro-CT scanning and the related parameters were calculated. HE staining was used to observe the pathological changes of rat femur. The protein expression level of JAK2/STAT3 signaling pathway was detected by Western Blot (WB). Results: (1) Pue significantly increased BMD in PMOP rats. (2) Micro-CT analysis showed that Pue could improve the bone microstructure of PMOP rats. (3) HE staining showed that after Pue treatment, the bone loss of PMOP rats decreased, the number and density of trabecular bone increased, and the pathological changes were restored. (4) WB results showed that compared with SHAM group, the expression levels of p-JAK2 and p-STAT3 protein in PMOP group were increased (P＜0.001). Compared with PMOP group, the expression levels of p-JAK2 and p-STAT3 protein in Pue-L group and Pue-H group were decreased (P＜0.001). Conclusion: Puerarin can effectively treat osteoporosis in PMOP rats, which may play an anti-osteoporosis role by inhibiting the activation of JAK2/STAT3 pathway.
ZHAO Xinlei
,
WANG Jianguo
,
LIU Youcai
,
ZHOU Jiaxuan
,
WANG Beiyu
,
LI Chang
,
ZHANG Yijun
. Mechanism of puerarin in the treatment of PMOP rats based on JAK2 / STAT3 signaling pathway[J]. Journal of Baotou Medical College, 2025
, 41(1)
: 25
-31
.
DOI: 10.16833/j.cnki.jbmc.2025.01.005
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