Objective: To explore the neurological deficit and behavioral changes of rats after cerebral ischemia-reperfusion injury. Methods: A total of 60 male Wistar rats were randomly divided into the Sham group, 24 h group, 48 h group, 72 h group, 7 d group and 14 d group, and the rat model of focal cerebral ischemia reperfusion injury (CIRI) was established using middle cerebral artery occlusion (MCAO). The neurological deficit of rats in each group was evaluated by modified neurological severity score (mNSS), and the weight of rats was monitored and recorded. The rate of cerebral infarction was detected by TTC staining, the sensory and motor abilities and emotional changes of rats in each group were evaluated by Adhesive Removal Test, Open Field Test and Rotarod Test. Results: After cerebral ischemia/reperfusion, the weight of rats decreased significantly (P＜0.01), especially at 72 h; Compared with the Sham group, the neurological deficit score of rats after cerebral ischemia-reperfusion increased significantly (P＜0.01), and the removal time of adhesive tape increased significantly (P＜0.01), reaching the peak at 48～72 h. The results of open field test showed that the moving distance of rats of the MCAO model was significantly decreased (P＜0.01), while the rest time was significantly increased (P＜0.01), with the significantly decreased average speed and standing time (P＜0.01), and rats of MCAO model were depressed, with the lowered curiosity and desire to explore the outside world. The results of rotarod test showed that the moving time and distance of rats in the MCAO model were decreased (P＜0.01), and the changes were more significant from 48～72 h. Conclusion: Cerebral ischemia-reperfusion injury after 48～72 h could weaken the neurological function and motor ability of rats, which seriously affect limb coordination and emotion of rats.
WANG Lu
,
ZHANG Shibin
,
LV Xue
,
JIA Jianxin
,
YANG Zhanjun
,
WU Li′e
. Changes of neurological function and behavior of rats after cerebral ischemia-reperfusion injury[J]. Journal of Baotou Medical College, 2025
, 41(1)
: 20
-24
.
DOI: 10.16833/j.cnki.jbmc.2025.01.004
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