Objective: To investigate the protective effect of Nox inhibitor APX-115 free base on H9c2 cells against high concentration of glucose. Methods: The proliferative activity of H9c2 cells after pretreatment with different concentrations of APX-115 free base was detected by CCK8 assay. The expression of Nox4 protein was detected by Western blot and immunofluorescence. The morphological changes of H9c2 cells were observed. Results: Compared with the Model group, whrn the concentration of APX-115 free base was 8 μmol/L, the cell proliferation activity decreased, and the cell proliferation activity increased at other concentrations. The expression of Nox4 protein in each treatment group of APX-115 free base was down-regulated. The cell morphology of the APX-115 free base 5 μmol/L treatment group returned to spindle shape, the cell distribution was gradually uniform, the intercellular space was reduced, and the number of cells basically returned to normal level. Conclusion: When APX-115 free base is 5 μmol/L, it has the best protective effect on H9c2 cells induced by high glucose.
WEI Xiaojuan
,
LI Shude
,
LI Zhigang
,
LI Qingling
,
HE Shuiwang
,
LI Siman
. Protective effect of APX-115 free base on H9c2 cells against high concentration of glucose[J]. Journal of Baotou Medical College, 2024
, 40(12)
: 8
-12
.
DOI: 10.16833/j.cnki.jbmc.2024.12.002
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