Objective: To investigate the role of cyclooxygenase-2 (COX-2) in the mechanism of skeletal muscle fibrosis and its relationship with Wnt/β-catenin signaling pathway. Methods: Seventy-two 20-week-old male C57 mice were randomly divided into normal group, model group and intervention group. The skeletal muscle injury model was established by fixed click method. The normal group was not modeled, and the model group and the intervention group were modeled. After successful modeling, the normal group and the model group were given 1mL normal saline daily by gavage, and the intervention group was given daily celecoxib (COX-2 specific inhibitor) by gavage at a dose of 100 mg/kg dissolved in 1ml normal saline. Each group was divided into 3 days, 7 days, 14 days, 21 days four subgroups (n=6) according to the sampling time. The expression of COX-2 and β-catenin in skeletal muscle tissue sections of each group was detected by immunohistochemical staining. Results: There was a small amount of COX-2 and β-catenin expression in skeletal muscle tissue of normal mice. Compared with the normal group, the expression of COX-2 and β-catenin in the model group was significantly increased (P＜0.01). Compared with the model group, the expression of COX-2 and β-catenin in the intervention group was significantly decreased (P＜0.01). Conclusion: COX-2 may affect the expression of β-catenin in skeletal muscle fibrosis by interfering with the expression of β-catenin.
LI Jia
,
DING Jian
,
CHEN Huan
,
MIAO Huachun
,
GONG Xin
,
DING Yanxia
. The mechanism of COX-2 in skeletal muscle fibrosis[J]. Journal of Baotou Medical College, 2024
, 40(11)
: 15
-18
.
DOI: 10.16833/j.cnki.jbmc.2024.11.004
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