Objective: To investigate whether intestinal fecal microbiota transplantation (FMT) can improve the efficacy of anti-programmed cell death protein 1 (PD-1) therapy in the treatment of ovarian cancer and its possible mechanisms of action. Methods: Twenty 5-week-old C57bl/6 SPF female mice were randomly divided into four groups, with five mice in each group, namely the control group (NC group), fecal bacteria transplantation FMT group, PD-1 group, and the combined treatment group (FMT combined with PD-1 group). Each group of mice was uniformly treated with broad-spectrum antibiotics for 2 weeks to construct a pseudo sterile mouse model. On day 0, ID8-luc cells were injected intraperitoneally to construct an ovarian cancer bearing mouse model. Before medication and 2 weeks after medication treatment, mice were subjected to one in vivo imaging system (IVIS) to evaluate the therapeutic effect. After the end of the experiment on the 30th day, the mice were decapitated and killed. Peritoneal tumor tissue was taken for HE and immunohistochemical staining to observe the pathological changes of the tumor tissue. Results: The combined treatment group was better than the single PD-1 group in treating ovarian cancer bearing mice, and reduced the expression of PD-L1 in tumor cells (P＜0.05),and increased the infiltration of CD8+T cells in the tumor microenvironment (TME) (P＜0.05). Conclusion: Fecal microbiota transplantation (FMT) enhances the efficacy of PD-1 monoclonal antibody in treating ovarian cancer bearing mice by increasing the infiltration of CD8+T cells in the tumor microenvironment (TME).
LU Wei
,
CHENG Zhong Ping
. Fecal microbiota transplantation improves the immune response of PD-1 mAb in the treatment of ovarian cancer[J]. Journal of Baotou Medical College, 2024
, 40(11)
: 5
-9
.
DOI: 10.16833/j.cnki.jbmc.2024.11.002
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