Objective: To investigate the effect of prone kneeling position in the second stage of labor on the delivery outcome of primipara who underwent labor analgesia. Methods: Base on the inclusion and exclusion methods, the primiparas who underwent labor analgesia were randomly divided into the observation group and the control group with 95 cases in each group. In the observation group, the prone kneeling position was adopted at the beginning of the second stage of labor until the fetus was exposed (S+4), and then the bladder lithotomy position was instead. The bladder lithotomy position was adopted in the control group for the whole of delivery.The women in both groups were delivered under conventional ways. The differences of labor and delivery outcomes between the two groups were analyzed and compared. Results: The second stage of labor in the observation group was shorter than that in the control group , and the one-minute Apgar score of newborns in one minute was higher than that in the control group. Binary logistic regression analysis showed that the vaginal delivery rate in the observation group was significantly higher than that in the control group (OR=8.644,95%CI 1.059-70.530), and the incidence of fetal deceleration during the second stage of labor was significantly lower than that in the control group (OR=0.253,95%CI 0.079-0.809). Conclusion: For primiparas who under going labor analgesia throughout the whole process of delivering, the application of prone kneeling position in the second stage of labor can shorten the second stage of labor, reduce the rate of conversion to cesarean section, increase the success rate of vaginal delivery, and improve Apgar score of newborns.