Objective: To explore the feasibility of non-invasive preimplantation genetic testing for aneuploidy (niPGT-A) using mouse blastocyst culture medium, and to provide theoretical basis and data support for optimizing and developing non-invasive preimplantation genetic screening technology. Methods: A total of 11 mouse blastocysts were collected. A small amount of trophectoderm cells (TE) were taken as the gold standard, inner cell mass (ICM) cells as the positive control, and blastocyst culture medium (BCM) as the sample to be verified. All embryo samples were analyzed for chromosome status based on whole genome sequencing data, and the chromosome ploidy consistency between different samples of the same embryo was compared. Results: In 11 mouse blastocysts, the detection rates of PGT-A in BCM, TE and ICM were 82.8% (9/11), 72.7% (8/11) and 100% (11/11), respectively. The chromosome consistency between BCM and TE, BCM and ICM, TE and ICM was 85.7% (6/7), 88.9% (8/9), 87.5% (7/9), respectively. There was no significant difference between any two groups (P＞0.05). Conclusion: In mouse model, noninvasive preimplantation genetic testing for aneuploidy using cell free DNA in blastocyst culture medium can obtain higher detection rate and more accurate chromosome detection results than trophoblastic ectoderm cells biopsy at the same time.
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