Objective: To explore the toxic effect of chronic fluorosis on myocardium and its effect on JNK-mediated apoptosis. Methods: Wistar rats were randomly divided into 4 groups: control group and different concentrations of sodium fluoride solution groups (50, 100, 200 mg·L-1 NaF). The rats were free to drink, and the control group drank deionized water. After 4 months, the rat model of chronic fluorosis was obtained. The effects of different concentrations of NaF on the myocardial tissue structure of rats were compared by HE staining, and the damage of myocardial tissue was observed. The expression of apoptosis-related proteins in myocardial tissue was detected by Western blot, and the effect of NaF on the expression of apoptotic proteins was observed, including p-JNK, Bcl-2, Bax and Caspase-3 protein expression. Results: After 4 months, a rat model of chronic fluorosis was established. The myocardial tissue of rats in each group showed muscle fiber structure disorder, muscle fiber breakage, muscle cell atrophy, muscle gap widening, and disappearance of transverse striations. The results of Western blot showed that compared with the control group, the expression of Bcl-2 protein in myocardium of rats with chronic fluorosis was significantly decreased (P＜0.05), and the expression of Bax, Caspase-3 and p-JNK protein was significantly up-regulated (P＜0.05). Conclusion: Chronic fluorosis may cause myocardial apoptosis by activating JNK signaling pathway, and apoptosis is involved in the damage of fluoride to myocardial tissue.
LI Tao
,
QI Reifang
,
SHI Jinghua
,
LYU Jun
,
WANG Lijun
,
Gao Yongsheng
,
MA Baohui
. Effects of fluoride exposure on myocardial apoptosis in rats[J]. Journal of Baotou Medical College, 2024
, 40(9)
: 13
-17
.
DOI: 10.16833/j.cnki.jbmc.2024.09.003
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