Objective:To optimize and improve the quality standard of Mongolian medicine Qintang powder. Methods: TLC (thin layer chromatography, TLC) was used for the qualitative identification of safflower and Wulingzhi in Qintang. HPLC (high performance liquid chromatography, HPLC)was used to determine the content of amentoflavone. The chromatographic separation was preformed on a C₁₈(250 mm ×4.6 mm, 5 μm) column with mobile phase of methanol -0.1% phosphoric acid. The detection wavelength was set at 330 nm, with the flow rate of 1.0 mL/min. Results: The TLC spots were clear without negative interference. The linear ranges of amentoflavone were 12.0～21.6 μg /mL, and the regression equation was y=119 894x-253 379 (R²=0.999 3). The average recovery rates were 104.5%, 102.9% and 103.5% respectively in high-, middle- and low recovery rate tests, with the RSD of 1.15%,1.63% and 0.28%. The content of amentoflavone was 0.012 84%～0.012 93%. Conclusion: The identification and content determination methods of Qintang powder established in this study are accurate and reliable, with high sensitivity, good repeatability, high precision and strong specificity, which can be used to optimize and improve the quality standard of Mongolian medicine Qintang powder.