Objective:To compare the effectiveness of fluorescence staining method and KOH wet mount method on detecting superficial fungal diseases and the detection rates at different detection sites. Methods: Specimens of patients who were diagnosed with superficial fungal disease at the Dermatology Department of the Second Affiliated Hospital of Baotou Medical College from October 2021 to March 2023 were collected. The fluorescence staining and KOH wet mount method were used for microscopic examination. The positive detection rate and the positive detection rate at different detection sites were compared. Results:The detection patients of the KOH wet mount method and fluorescence staining method were 558 and 691 cases (the positive rates were 38.35% and 58.47%) respectively, with a statistically significant difference in positive detection rates (χ2=49.967, P<0.01). And the positive detection rate of fluorescence staining at the head, face, hands, feet, trunk, groin, and nail parts was higher than that of KOH wet mount method, and the differences were statistically significant. The differences were sequentially decreased from foot, head, face, groin, hand, nail, and trunk parts. Conclusion: The positive detection rate of fluorescence staining tests on superficial fungal diseases is significantly better than that of KOH wet mount method, which could reduce the missed and misdiagnosed rate. It is preferred to detect superficial fungal diseases at nail, foot, groin, head and face, while hand and body detection could choose KOH test or fluorescence staining test based on the clinical experience of doctors to relieve the economic burden of patients.
YANG Meili
,
WU Junying
,
YANG Huijuan
,
LI Danyang
,
ZHAO Yiyuan
,
Jimusi
,
HAO Yong
,
SHI Jihai
. Comparative study between fluorescence staining method and KOH wet mount method on 1 249 cases of superficial mycosis in Baotou area[J]. Journal of Baotou Medical College, 2024
, 40(7)
: 53
-56
.
DOI: 10.16833/j.cnki.jbmc.2024.07.009
[1] 王俊霖, 黄云丽, 董天祥, 等. 昆明地区3524例浅部真菌感染病原学分析[J]. 皮肤病与性病, 2020, 42(2): 169-170.
[2] Nielsen PR, Eriksen JO, Lindahl LM, et al. Diagnostic two-gene classifier in early-stage mycosis fungoides: a retrospective multicenter study[J]. J Invest Dermatol, 2021, 141(1): 213-217.
[3] Tawfik KM, Mohammed RR, Sobhy A, et al. Conventional and molecular diagnostics for detection of causative fungi in clinical samples of human dermatomycosis[J]. J Egyptian Women's Dermatol Soc, 2022, 19(1): 21-30.
[4] 赵琳, 乔昀. 荧光染色法在真菌检测中的应用[J]. 检验医学, 2021, 36(12): 1219-1221.
[5] 杨颜龙, 张素敏, 易松柏, 等. 荧光染色法和氢氧化钾湿片法在诊断浅部真菌感染中的敏感度比较[J]. 中国麻风皮肤病杂志, 2019, 35(7): 389-393.
[6] 杜鹏, 黄嘉怡, 沈守星, 等. 荧光染色法和KOH湿片法、真菌培养法在皮肤浅部真菌感染诊断中的应用比较[J]. 中国麻风皮肤病杂志, 2018, 34(12): 712-714.
[7] 郝迪, 陈萍, 王静, 等. 儿童浅部真菌病1586例荧光染色法和KOH湿片法镜检效果比较[J]. 皮肤性病诊疗学杂志, 2022, 36(10): 1149-1154.
[8] 李晓晓, 李志瑜. 莱芙特敏荧光染色法、Baso荧光染色法与KOH湿片法在诊断浅部真菌病的应用对比[J]. 中国真菌学杂志, 2020, 15(2): 111-113.
[9] 谢振谋, 黄进梅, 刘红芳, 等. 4018例浅部真菌病及其致病菌种分析[J]. 皮肤性病诊疗学杂志, 2022, 29(4): 337-341.
[10] Gupta AK, Taborda VBA, Taborda PRO, et al. High prevalence of mixed infections in global onychomycosis[J]. PloS one, 2020, 15(9): e0239648.
[11] 甲真菌病指南专家工作组. 中国甲真菌病诊疗指南(2021年版)[J]. 中国真菌学杂志, 2022, 17(1): 1-7.