Objective: To explore the correlation between ALEX1 level and clinical features of breast cancer histopathologic detection by immunohistochemistry. Methods: ALEX1 positive rate, protein and mRNA levels were detected by immunohistochemistry, western blotting and PCR in 77 cases of breast cancer and 2 cm adjacent specimens, and the correlation between ALEX1 and clinicopathologic features was analyzed. Results: ALEX1 showed yellow and yellowish brown expression in the cell membrane of 77 breast cancer tissues and adjacent tissues. ALEX1 positive expression in 45 breast cancer tissues was 58.44%, and positive rate in 61 adjacent tissues was 79.22%. There was significant difference between groups (χ2=4.199, P＜0.05). The protein expression of ALEX1 in 77 breast cancer tissues and adjacent tissues was 2.05±0.55 and 4.11±0.69, respectively. The protein content of ALEX1 in breast cancer tissues was lower than that in adjacent tissues, and there was significant difference between groups (t=20.85, P＜0.001). The expression of ALEX1mRNA was 1.00±0.00 and 0.45±0.08 in 77 paracancer tissues and breast tissues, and there were significant differences in the expression of ALEX1mRNA in different tissues (t=60.330,P＜0.001). ALEX1 expression was correlated with pathological type, pathological grade and lymph node metastasis (P＜0.05), but not with other indicators (P＞0.05). The relationship between ALEX1 level and clinicopathology was observed according to the 3 classification boundaries. The decreased level of ALEX1 was correlated with invasive ductal carcinoma and pathological grade Ⅱ to Ⅲ of breast cancer patients (P＜0.05). The AUC area of ALEX1 in breast cancer was 0.646, and the specificity and sensitivity were 55.42% and 65.02%, respectively, indicating the significance of ALEX1 expression detection in the pathological diagnosis of breast cancer. Conclusion: The expression level of ALEX1 in paracancer tissues is significantly higher than that in breast cancer tissues, and the decreased expression of ALEX1 is associated with increased lymph node metastasis and pathological grade of breast cancer.
LIU Qirong
,
WU Yuqiang
,
YUE Guihua
,
DENG Xiaoling
,
LUO Yuan
. Analysis of correlation between ALEX1 level and clinical features in breast cancer tissues[J]. Journal of Baotou Medical College, 2024
, 40(6)
: 61
-65
.
DOI: 10.16833/j.cnki.jbmc.2024.06.011
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