Objective: To explore whether the glycosides of cistanche can improve the learning and cognitive function of sleep deprivation model rats by regulating synaptic plasticity. Methods: Fifty male Wistar rats were randomly divided into blank control group (Control), platform control group (PC), model group (Model), glycosides of cistanche group (GCs) and estazolam group (Est). The sleep deprivation model of rats was prepared by modified multi-platform water environment method. Morris water maze and open field test were used to detect the spatial cognitive function and emotional changes of rats. Nissl staining was used to observe the changes of morphology and quantity of nerve cells in hippocampal CA1 area of rats. Western blot and RT-PCR were used to detect the expression levels of synaptophysin (SYN), postsynaptic membrane dense substance 95 (PSD-95) and brain-derived neurotrophic factor (BDNF). Results: Morris water maze results showed that compared with the Control group, the learning and cognitive ability of rats in the Model group was significantly decreased (P＜0.05), and the learning and memory function of rats was improved after GCs treatment (P＜0.05). The results of the open field test showed that compared with the Control group, the movement distance and standing times of the Model group were significantly increased (P＜0.05), the resting time was significantly shortened(P＜0.05), and the anxiety was increased, while after GCs treatment, the movement distance and standing times decreased (P＜0.05), the resting time increased (P＜0.05), and the anxiety was improved. The results of Western blot and RT-PCR showed that the protein and gene expression levels of BDNF, SYN and PSD-95 in the Model group were significantly decreased (P＜0.05), while after treatment with GCs, the expression levels of BDNF, SYN and PSD-95 were significantly up-regulated (P＜0.05). Conclusion: Glycosides of cistanche may affect synaptic plasticity by regulating the expression of synaptic related markers, thereby improving the learning and cognitive function of sleep deprivation model rats.
ZHANG Shibin
,
WANG Lu
,
LI Hao
,
SUN Yihong
,
SONG Wei
,
YAN Xusheng
,
WANG Yanguo
,
JIA Jianxin
. Effects of glycosides of cistanche on learning cognition and synaptic plasticity in sleep deprivation model rats[J]. Journal of Baotou Medical College, 2024
, 40(6)
: 14
-19
.
DOI: 10.16833/j.cnki.jbmc.2024.06.003
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