Objective: To investigate the effects of helicid (HEL) on cognitive function of Aβ1-42 induced Alzheimer's disease (AD) rats. Methods: Twenty-four healthy male SD rats were randomly divided into Normal saline(NS) group,Aβ1-42+NS group,Aβ1-42+HEL group and HEL group,with 6 rats in each group. The AD model was established by injecting Aβ1-42(5 μg/μL) into the hippocampus of brain stereotaxic injection . After 3 days of injection, Aβ1-42+NS group and Aβ1-42+HEL group were given normal saline and Helicid(50 mg/kg) for 4 weeks. NS group and HEL group were given normal saline and Helicid(50 mg/kg) for 4 weeks,respectively. After treatment, the behavior and cognitive ability of rats in each group were evaluated by Morris water maze test. HE staining was used to detect the morphological changes of Hippocampal CA1 cells in each group. The expression of nuclear factor-κB (NF-κB) in hippocampal CA1 region was detected by Immunohistochemistry. Western blot was used to detect the protein expressions of (amyloiol protein precursor,APP), NF-κB and TNF-α in Hippocampal CA1. Results: Aβ1-42+NS group's escape latency was increased significantly more than the NS group, through the original times and stay in the original platform quadrant were significantly decreased. Hippocampal CA1 cell morphology showed obvious pyknosis; The expressions of APP, NF-κB and TNF-α were obviously increased(P＜0.05). Compared with the Aβ1-42+NS group, the escape latency of rats in the Aβ1-42+HEL group was obviously decreased, the number of crossing the platform and the residence time in the target quadrant were significantly increased. Pyknosis of nucleus in Hippocampal CA1 was improved. The expressions of APP, NF-κB and TNF-α were obviously decreased (P＜0.05). There was no significant difference in Morris water maze results between NS group and HEL group. APP, NF-κB and TNF-α proteins had no distinct changes in Hippocampal CA1. Conclusions: Helicid can improve the cognitive function of AD rats induced by Aβ1-42,which may be related to the inhibiting the expression of APP, NF-κB and TNF-α proteins.
WANG Qinqin
,
LUO Xiaoxiao
,
ZHAO Jian
,
DING Jian
,
WANG Mengya
,
ZHENG Lanrong
,
ZHENG Chao
. Effect of Helicid on cognitive function in Aβ1-42 induced Alzheimer's disease model rats[J]. Journal of Baotou Medical College, 2024
, 40(5)
: 12
-16
.
DOI: 10.16833/j.cnki.jbmc.2024.05.003
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