Objective: To explore the diagnostic and prognostic value of FAM25A in pancreatic cancer by bioinformatics methods, analyze its correlation with immune infiltration, and elaborate the potential mechanism of FAM25A in the occurrence and development of pancreatic cancer. Methods: The Pancreatic cancer datasets were obtained from TCGA, GTEx and GEO databases, and Rstudio was used for data analysis and visualization. The expression difference of FAM25A was clarified. The relationship between the expression of FAM25A and the overall survival time of pancreatic cancer was analyzed by K-M. The correlation between FAM25 A expression and prognostic factors of pancreatic cancer was analyzed by Cox regression. The correlation between FAM25A expression and immune cell infiltration was analyzed. According to WGCNA, the module genes most related to FAM25A and tumor purity were imported into STRING to construct PPI network and obtain GO and KEGG. GSEA was used to analyze the enrichment pathway of high and low expression of FAM25A, and MOCDE clustering subnetwork in Cytoscape was used to obtain significantly related nodes. Based on the above information, the potential mechanism of FAM25A in pancreatic cancer was analyzed. Results: The results showed that FAM25A was significantly more highly expressed in pancreatic cancer (P＜0.01); K-M analysis showed that the survival time of patients with high expression of FAM25 A was significantly shorter than that of patients with low expression; Cox regression analysis of prognostic factors related to pancreatic cancer suggested that FAM25A was an independent risk factor for pancreatic cancer (HR=2.4, 95%CI=1.614-3.592); Correlation analysis of immune cell infiltration showed that FAM25A could reduce T lymphocyte level; GO, KEGG, GSEA and MCODE results showed that FAM25A mainly played a role in cell-cell interaction, intercellular signal generation and transduction, chemokine and immune response. Conclusion: FAM25A over-expression in pancreatic cancer is an independent poor prognostic factor in pancreatic cancer. Abnormal expression of FAM25A can also be used as bio-markers for diagnosis and prognosis of pancreatic cancer. Its biological effects may be achieved by promoting proliferation, invasion and metastasis of pancreatic cancer.
ZHAO Yihao
,
MA Xingyan
,
ZHANG Dongbin
. Bioinformatics analysis of clinical value and potential mechanism of FAM25A in pancreatic cancer[J]. Journal of Baotou Medical College, 2024
, 40(4)
: 59
-66
.
DOI: 10.16833/j.cnki.jbmc.2024.04.011
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