Objective: To investigate the expression of miR-17-5p in lung adenocarcinoma cells, and to study the effects of miR-17-5p on proliferation, invasion, migration and apoptosis of lung adenocarcinoma cells and its molecular mechanism. Methods: The expression of miR-17-5p in human lung adenocarcinoma cell line HCC827 and human normal lung epithelial cell line BEAS-2B were detected by real-time fluorescent quantitative PCR. There were miR-17-5p mimics group, miR-17-5p inhibitor group and NC group in the experiment. The effects of miR-17-5p on proliferation, apoptosis, invasion and migration of lung adenocarcinoma cells were analyzed by CCK-8, flow cytometry and Transwell assay. Finally, PKD2 was predicted to be a potential downstream target gene of miR-17-5p by Targetscan database. The interaction between miR-17-5p and PKD2 was verified by real-time fluorescence quantitative PCR and Western-blot experiments. Results: miR-17-5p was highly expressed in HCC827 cells (P＜0.001), and up-regulation of miR-17-5p significantly promoted the proliferation (P＜0.05 ), invasion (P＜0.01 ) and migration (P＜0.05) of lung adenocarcinoma cells, and inhibited apoptosis (P＜0.01 ). There was a direct binding site between miR-17-5p and 3'UTR of PKD2, and overexpression of miR-17-5p significantly promoted the expression of PKD2 at both mRNA and protein levels (P＜0.01). Conclusion: PKD2 was the direct target gene of miR-17-5p, and miR-17-5p affected the biological behavior of lung adenocarcinoma cells by regulating PKD2.
CHEN Xiaoyan
,
LIU Jingyi
,
NIU Haiying
,
SUN Jianfang
,
HE Huijie
,
ZHANG Dong
. The effect of miR-17-5p on biological behavior of lung adenocarcinoma HCC827 cells by regulating PKD2[J]. Journal of Baotou Medical College, 2024
, 40(4)
: 8
-14
.
DOI: 10.16833/j.cnki.jbmc.2024.04.002
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