Objective: To study the interaction of taurine with hepatic proteins in rats with cirrhotic portal hypertension and its underlying mechanism using spectroscopy. Methods: A total of 120 rats were randomly divided into the normal group, model group, propranolol group and taurine group, with 30 rats in each group. Except for the normal group, the other three groups were treated with carbon tetrachloride (CCL4) to prepare a cirrhotic portal hypertension rat model. When modeling, the propranolol group was given propranolol, the taurine group was treated with taurine, and rats in the model group were fed with the same amount of normal saline. All rats were treated for a course of 10 weeks. After treatment, values of portal hypertension in each group were compared. The fluorescence spectra of hepatic protein and the ultraviolet absorption spectrum of taurine of rats in each group at different temperatures were measured and analyzed. Results: Compared to the propranolol group and model group, portal venous flow (PVF), portal venous pressure (PVP), portal vascular resistance (PVR) and heart rate were lower and mean arterial pressure (MAP) was higher in the taurine group. Kq values of hepatic protein for each rats were greater than the maximum dynamic quenching rate of 2.0×1010L/(mol·s), with the enthalpy change(△H)of -8707.6336 and entropy change(△S)of 33.41395. △G and △H of Gibbs free energy change in the normal group were both negative, and △S was positive. △G and △H in the taurine group were positive, with negative △S, while the model group had no obvious regularity. The R values were 1.77, 2.35, and 2.28 nm in the normal, model and taurine group respectively, with the r values of 1.24, 1.31 and 6.56 respectively. Conclusion: Taurine can inhibit fibrosis and attenuate portal hypertension, and this mechanism may alter the protein structure of liver tissue in cirrhotic portal hypertension rats to alleviate liver damage.
YU Ye
,
LIAO Juan
,
WEN Bin
,
DENG Xin
. Study on the interaction of taurine and hepatic protein in rats with cirrhotic portal hypertension by spectrometry[J]. Journal of Baotou Medical College, 2024
, 40(3)
: 42
-49
.
DOI: 10.16833/j.cnki.jbmc.2024.03.008
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