Objective: To study the changes of phenylalanine metabolites in serum of diabetic kidney disease (DKD) patients and healthy controls using targeted metabolomics, and to comprehensively analyze the change rule of differential metabolites in serum and its correlation with clinical indicators. Methods: The inpatients in the Department of Nephrology of the First Affiliated Hospital of Baotou Medical College were selected as the research subjects. 20 DKD patients were selected as the study group and 20 healthy subjects as the control group. Blood samples were collected from the two groups. The difference of serum metabolites was analyzed by liquid chromatography/mass spectrometry (LC/MS) and targeted metabolomics. Univariate and multivariate analysis and statistical analysis were performed to analyze the difference of serum metabolites and clarify their significance in DKD. Results: Serum levels of Phenylalanine metabolites (L-phenylalanine, Hippuric acid, D-phenlacetyl-L-glutamine) in DKD patients were significantly higher than those in control group. The enrichment analysis of the pathway involved in serum metabolites showed that phenylalanine metabolism pathway was involved in the progress of DKD. Serum metabolites of phenylalanine metabolism pathway in DKD patients, including L-phenylalanine, Hippuric acid and D-phenlacetyl-L-glutamine, were positively correlated with Scr, FBG and HbA1c. Conclusion: Phenylalanine metabolism pathway may be involved in the occurrence and development of DKD.
YANG Jie
,
MI Yan
,
WANG Caili
. Analysis on the changes of serum phenylalanine metabolites in diabetic renal disease based on targeted metabolomics[J]. Journal of Baotou Medical College, 2024
, 40(3)
: 21
-28
.
DOI: 10.16833/j.cnki.jbmc.2024.03.005
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