Objective: To investigate the effect and mechanism of remote ischemic preconditioning (RIPC) separating medium on hypoxia/reoxygenation (H/R) injury of human renal tubular epithelial cells (HK-2). Methods: HK-2 cells were divided into time control group, H/R, RIPC treatment group and autophagy intervention group. Cells in the time control group were cultured under normal conditions. Cells in the RIPC group were pretreated with RIPC separating medium. Cells in the autophagy intervention group were treated with autophagy inhibitor 3-methyladenine (3MA) on the basis of RIPC treatment. Cells in the latter three groups were subjected to H/R in a three-gas incubator to construct a damage model. After the success of the model, the cell status of each group was observed by optical microscope, and the expression levels of LC3Ⅱ/Ⅰ protein and Beclin1 protein were observed by Western blot. Results: Under the microscope, RIPC separating medium could significantly reduce the damage of H/R to HK-2 cells. After adding autophagy inhibitor, the protective effect of RIPC separating medium on H/R injury of HK-2 cells was blocked. The results of Western blot showed that RIPC separating medium could increase the expression levels of autophagy-specific proteins LC3Ⅱ/Ⅰ and Beclin1 in mitochondria of HK-2 cells. The results of Real-time PCR showed that the mRNA expression of LC3 and Beclin1 in RIPC group was significantly higher than that in H/R group; the expression of autophagy-specific proteins LC3Ⅱ/Ⅰ and Beclin1 in mitochondria of HK-2 cells was decreased by adding autophagy inhibitors, and the mRNA expression of LC3 and Beclin1 was also significantly decreased. Conclusion: RIPC separating medium has a protective effect on HK-2 cells after H/R injury, and the mechanism may be related to mitophagy.
DAI Wenjun
,
PEI Hanjun
. Protective effect of remote ischemic preconditioning separating medium on hypoxic injury of human renal tubular epithelial cells[J]. Journal of Baotou Medical College, 2024
, 40(2)
: 87
-92
.
DOI: 10.16833/j.cnki.jbmc.2024.02.016
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