Objective: To prepare the targeted lipid-microbubble ultrasonographic contrastagent loaded with paclitaxel LHRH, and to evaluate its physical and chemical properties,Methods:LHRH targeted lipid-microbubble was prepared by Co-evaporating method, mechanical vibration method and biotin-avidin method. The size and surface electric potential were detected by Malvern detector and the imaging was observed by ultrasonography. The drug-loading capacity and encapsulation rate were determined by high performance liquid chromatography (HPLC). The connection rates of secondary antibody with targeted and non-targeted lipid-microbubble were detected by flow cytometry (FCM).Results: The targeted lipid- microbubble had complete appearance and even distribution. After being kept at room temperature for 14 days, there was no significant difference of general properties ( P＞0.05). The binding rates of secondary antibody with targeted and non-targeted lipid-microbubble were 97.76±2.18 % and 6.48±0.71 % respectively, which had significant difference (P＜0.05).Conclusion: The targeted lipid-microbubble loaded with paclitaxel is successfully prepared, with smaller size and higher stability. It has a high connection rate of LHRH.
MA Lan
,
YAN Guozhen
,
HE Junfeng
,
WEN Qing
. Study in preparation of lipid-microbubble ultrasonographic contrastagent loaded with paclitaxel LHRH and its properties[J]. Journal of Baotou Medical College, 2017
, 33(9)
: 129
-131
.
DOI: 10.16833/j.cnki.jbmc.2017.09.054
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