CONTENTS IN BRIEF

Study in preparation of lipid-microbubble ultrasonographic contrastagent loaded with paclitaxel LHRH and its properties

  • MA Lan ,
  • YAN Guozhen ,
  • HE Junfeng ,
  • WEN Qing
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  • 1.Graduate School of Baotou Medical College, Baotou,Inner Mongolia,014060 China;
    2.Department of ultrasound, the First Affiliated Hospital of Baotou Medical College,Baotou,Inner Mongolia,014010 China

Received date: 2017-07-07

  Online published: 2017-09-26

Abstract

Objective: To prepare the targeted lipid-microbubble ultrasonographic contrastagent loaded with paclitaxel LHRH, and to evaluate its physical and chemical properties,Methods:LHRH targeted lipid-microbubble was prepared by Co-evaporating method, mechanical vibration method and biotin-avidin method. The size and surface electric potential were detected by Malvern detector and the imaging was observed by ultrasonography. The drug-loading capacity and encapsulation rate were determined by high performance liquid chromatography (HPLC). The connection rates of secondary antibody with targeted and non-targeted lipid-microbubble were detected by flow cytometry (FCM).Results: The targeted lipid- microbubble had complete appearance and even distribution. After being kept at room temperature for 14 days, there was no significant difference of general properties ( P>0.05). The binding rates of secondary antibody with targeted and non-targeted lipid-microbubble were 97.76±2.18 % and 6.48±0.71 % respectively, which had significant difference (P<0.05).Conclusion: The targeted lipid-microbubble loaded with paclitaxel is successfully prepared, with smaller size and higher stability. It has a high connection rate of LHRH.

Cite this article

MA Lan , YAN Guozhen , HE Junfeng , WEN Qing . Study in preparation of lipid-microbubble ultrasonographic contrastagent loaded with paclitaxel LHRH and its properties[J]. Journal of Baotou Medical College, 2017 , 33(9) : 129 -131 . DOI: 10.16833/j.cnki.jbmc.2017.09.054

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