Objective: To study the immunoregulatory effect of umbilical cord mesenchymal stem cells (UC-MSCs) on peripheral blood of patients with Sjogren's syndrome (SS). Methods: UC-MSCs were isolated and cultured from umbilical cord tissue of newborns, and the cell surface markers of UC-MSCs were detected by flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from 20 SS patients in the active phase of the disease. Co-cultures of PBMCs from SS patients with UC-MSCs were established, and cell viability was assessed using the CCK-8 assay. The optimal ratio of co-cultured cells was determined for subsequent experiments. Flow cytometry was used to analyze the changes in lymphocyte subsets in SS patient PBMCs after co-culture. Results: UC-MSCs reduced the proportions of peripheral blood CD3+ T cells, CD3+ CD8+ T cells, and CD19+ B cells in PBMC of SS patients, while the proportions of CD16+ CD56+ NK cells, CD3+ CD4+ T cells, and CD4+ /CD8+ ratio were not significantly affected. Conclusion: UC-MSCs can modulate the immune function of SS patients, thereby exerting a certain therapeutic effect on SS.
WANG Sibo
,
WU Tao
,
PANG Chunyan
,
WANG Hui
. Study on immune regulation of umbilical cord mesenchymal stem cells in peripheral blood of patients with Sjogren's syndrome[J]. Journal of Baotou Medical College, 2024
, 40(2)
: 28
-31
.
DOI: 10.16833/j.cnki.jbmc.2024.02.005
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