Establishment and verification of a renal clear-cell carcinoma tumor prognosis-model based on autophagy and ferroptosis genes

  • LI Yuqi ,
  • LUO Qian ,
  • KUANG Yunshu ,
  • LIANG Xiao ,
  • LU Guangtao ,
  • ZHU Xiaoqun ,
  • CHEN Bing
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  • Pathology Teaching and Research Office, Wannan Medical College, Wuhu 241002, China

Received date: 2022-12-05

  Online published: 2024-01-09

Abstract

Objective: To investigate the influence of autophagy-relevant genes (ARGs) and ferroptosis-related genes (FRGs) on the prognosis of patients with ccRCC(clear cell renal carcinoma), and evaluate the prognosis based on ARGs and FRGs models of ccRCC. Methods: Risk-related ARGs and FRGs were identified in the ccRCC dataset from the TCGA database, and functional enrichment analysis and tumor classification were performed. The Cox proportional hazards model of 37 ccRCC patients was established through univariate and multivariate Cox regression. Multi-index ROC curve was used to assess the accuracy of the model. Finally, a GSE29609 dataset was used to verify whether our conclusion is feasible or not. Results: A total of 37 differentially expressed ARGs and FRGs were found. Univariate and multivariate Cox regression identified 8 risk-related genes related to OS, which are CASP4, PRKCQ, BNIP3,BAG1,BIRC5,CHAC1,ATG16L2 and EIF4EBP1. A Kaplan-Meier analysis results showed that patients at high risk of morbidity had lower survivability. The area (performance index) under the ROC curve was >0.75, indicating that the model’s prediction accuracy was higher. Then the immune cell infiltration were evaluated based on the CIBERSORT algorithm. Conclusion: The prognostic model of ccRCC-related genes based on 8 ARGs and FRGs has a certain accuracy and could be used to guide clinical treatment more accurately.

Cite this article

LI Yuqi , LUO Qian , KUANG Yunshu , LIANG Xiao , LU Guangtao , ZHU Xiaoqun , CHEN Bing . Establishment and verification of a renal clear-cell carcinoma tumor prognosis-model based on autophagy and ferroptosis genes[J]. Journal of Baotou Medical College, 2024 , 40(1) : 21 -29 . DOI: 10.16833/j.cnki.jbmc.2024.01.004

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